Domain

Glycosyl-hydrolase family 116, catalytic region (IPR006775)

Short name: GH116_catalytic

Domain relationships

None.

Description

This entry represents the catalytic region found in the CAZyme GH116 family members, which presently includes enzymes with beta-glucosidase (EC:3.2.1.21), beta-xylosidase (EC:3.2.1.37) , and glucocerebrosidase (EC:3.2.1.45) activity [PMID: 20427274]. Proteins containing this domain include animal non-lysosomal glucosylceramidase GBA2, which catalyse the conversion of glucosylceramide to free glucose and ceramide [PMID: 17105727]. GBA2 is involved in sphingomyelin generation and prevention of glycolipid accumulation and may also catalyse the hydrolysis of bile acid 3-O-glucosides, however, the relevance of such activity is unclear in vivo [PMID: 17080196]. Mutations in the human protein cause motor-neurone defects in hereditary spastic paraplegia [PMID: 23332917]. The catalytic nucleophile, identified in Q97YG8 is a glutamine-335, with the likely acid/base at Asp-442 and the aspartates at Asp-406 and Asp-458 residues also playing a role in the catalysis of glucosides and xylosides that are beta-bound to hydrophobic groups [PMID: 20427274].

GO terms

Biological Process

No terms assigned in this category.

Molecular Function

GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
Pfam