Domain

EB1, C-terminal (IPR004953)

Short name: EB1_C

Domain relationships

None.

Description

A group of microtubule-associated proteins called +TIPs (plus end tracking proteins), including EB1 (end-binding protein 1) family proteins, label growing microtubules ends specifically in diverse organisms and are implicated in spindle dynamics, chromosome segregation, and directing microtubules toward cortical sites. EB1 members have a bipartite composition: the N-terminal CH domain (IPR001715) mediates microtubule plus end localization and a C-terminal cargo binding domain (EB1-C) that captures cell polarity determinants. The EB1-C domain comprises a unique EB1-like sequence motif that acts as a binding site for other +TIP proteins. It interacts with the carboxy terminus of the adenomatous polyposis coli (APC) tumor suppressor, a well conserved +TIP phosphoprotein with a pivotal function in cell cycle regulation. Another binding partner of the EB1-C domain is the well conserved +TIP protein dynactin, a component of the large cytoplasmic dynein/dynactin complex [PMID: 14614826, PMID: 15699215, PMID: 15616574].

The ~80-residue EB1-C domain starts with a long smoothly curved helix (alpha1), which is followed by a hairpin connection leading to a short second helix (alpha2) running antiparallel to alpha1. The two parallel alpha1 helices of the EB1-C domain dimer wrap around each other in a slightly left-handed supercoil. The two alpha2 helices run antiparallel to helices alpha1 and form a similar fork in the opposite orientation and rotated by 90 degrees. As a result, two helical segments from each monomer form a four-helix bundle. The side chain forming the hydrophobic core of this bundle are highly conserved [PMID: 15699215, PMID: 15616574, PMID: 16109370].

Some protein known to contain an EB1-C domain are listed below:

  • Yeast protein BIM1.
  • Fission yeast microtubule integrity protein mal3.
  • Vertebrate microtubule-associated protein RP/EB family member 1 (EB1).
  • Vertebrate microtubule-associated protein RP/EB family member 2 (EB2 or RP1).
  • Vertebrate microtubule-associated protein RP/EB family member 3 (EBF3).

GO terms

Biological Process

No terms assigned in this category.

Molecular Function

GO:0008017 microtubule binding

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
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