Ribonuclease H2, subunit A (IPR004649)

Short name: RNase_H2_suA

Overlapping homologous superfamilies

Family relationships


Whereas bacterial and archaeal RNases H2 are active as single polypeptides, the Saccharomyces cerevisiae (Baker's yeast) homologue, Rnh2Ap, when expressed in Escherichia coli, fails to produce an active RNase H2. For RNase H2 activity three proteins are required [Rnh2Ap (Rnh201p), Ydr279p (Rnh202p) and Ylr154p (Rnh203p)]. Deletion of any one of the proteins or mutations in the catalytic site in Rnh2A leads to loss of RNase H2 activity [PMID: 14734815]. RNase H2 is an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. It participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication.

This entry represents the catalytic chain of RNase H2, which is found as a single polypeptide in prokaryotes and is part of a three protein complex in eukaryotes. In Saccharomyces cerevisiae (Baker's yeast) it is represented by P53942.

GO terms

Biological Process

GO:0016070 RNA metabolic process

Molecular Function

GO:0004523 RNA-DNA hybrid ribonuclease activity

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.