Alkylated DNA repair protein AlkB (IPR004574)

Short name: Alkb

Overlapping homologous superfamilies

Family relationships



AlkB proteins are dioxygenases that repair alkylation damage to DNA and RNA [PMID: 12594517]. The Escherichia coli alkB gene product protects against cell killing by S(N)2-alkylating agents through DNA repair by a novel direct reversal DNA repair mechanism: the oxidative demethylation of N1-methyladenine or N3-methylcytosine DNA lesions. This reaction occurs on both single- and double-stranded DNA, and requires AlkB-bound non-heme Fe(2+), O(2) and alpha-ketogluterate to oxidize the offending methyl group. This is followed by the release of succinate, CO(2) and formaldehyde, and the restoration of undamaged A or C in DNA [PMID: 12517444].

Nine mammalian AlkB homologues exist (ALKBH1-8, FTO), but only a subset functions as DNA/RNA repair enzymes [PMID: 26152727], and only ALKBH1 is included in this entry. In humans, ALKBH1 (ABH1) is responsible for the conversion of 5-methylcytosine to 5-formylcytosine (f5C) in position 34 in mitochondrial tRNAMet [PMID: 28257121].

GO terms

Biological Process

GO:0055114 oxidation-reduction process

Molecular Function

No terms assigned in this category.

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.