Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, B subunit (IPR004413)

Short name: Apn/Gln-ADT_bsu

Overlapping homologous superfamilies

Family relationships


Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase (EC:6.3.5.-) allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) [PMID: 9342321]. The enzyme is composed of three subunits: A (an amidase), B and C. It also exists in eukaryotes as a protein targeted to the mitochondria.

The heterotrimer GatABC is involved in converting Glu to Gln and/or Asp to Asn, when the amino acid is attached to the appropriate tRNA. In Lactobacillus, GatABC is responsible for producing tRNA(Gln). In Archaea, GatABC is responsible for producing tRNA(Asn), while GatDE is responsible for producing tRNA(Gln). In lineages that include Thermus, Chlamydia, or Acidithiobacillus, the GatABC complex catalyses both tRNA(Gln) and tRNA(Asn).

This entry represents the B subunit of aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase.

GO terms

Biological Process

No terms assigned in this category.

Molecular Function

GO:0016884 carbon-nitrogen ligase activity, with glutamine as amido-N-donor

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.