Domain

DhaL domain (IPR004007)

Short name: DhaL_dom

Domain relationships

Description

Dihydroxyacetone (Dha) kinases are a family of sequence-conserved enzymes that phosphorylate dihydroxyacetone, glyceraldehyde and other short-chain ketoses and aldoses. They can be divided into two groups according to the source of high-energy phosphate that they utilise, either ATP or phosphoenolpyruvate (PEP). The ATP-dependent forms are the two-domain Dha kinases (DAK), which occur in animals, plants and eubacteria. They consist of a Dha binding (K) and an ATP binding (L) domain. The PEP-dependent forms occur only in eubacteria and a few archaebacteria and consist of three subunits. Two subunits, DhaK and DhaL, are homologous to the K and L domains. Intriguingly, the ADP moiety is not exchanged for ATP but remains permanently bound to the DhaL subunit where it is rephosphorylated in situ by the third subunit, DhaM, which is homologous to the IIA domain of the mannose transporter of the bacterial PEP:sugar phosphotransferase system (PTS) [PMID: 16647083, PMID: 18957416].

The DhaL domain consists of eight antiparallel alpha-helices arranged in an up-and-down geometry and aligned on a circle. This results in the formation of a helix barrel enclosing a deep pocket. The helices are amphipathic with the hydrophobic side chains directed into the pocket of the barrel and with the polar residues exposed. The nucleotide is bound on the top of the barrel [PMID: 16647083, PMID: 12966101].

The DhaL alpha helix barrel fold appears not only as C-terminal domain in Dha kinases but also as N-terminal domain in a family of two-domain proteins with unknown function. One representative example is YfhG of Lactococcus lactis [PMID: 12966101].

GO terms

Biological Process

GO:0006071 glycerol metabolic process

Molecular Function

GO:0004371 glycerone kinase activity

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE profiles
Pfam
SUPERFAMILY