Bifunctional nuclease domain (IPR003729)

Short name: Bi_nuclease_dom

Overlapping homologous superfamilies

Domain relationships



The bifunctional nuclease (BFN) domain is specific to bacteria and plant organisms. It has both RNase and DNase activities [PMID: 20018603].

The dimer of the BFN domain forms a wedge, each monomer being a basic triangular shape. The BFN domain is composed of an eight-stranded, distorted beta-sheet consisting of a four-stranded, antiparallel beta-sheet (B1, B2, B3, B8), and a four-stranded mixed beta-sheet (B4, B5, B6, B7). The sheets are intercalated by three short alpha-helices (H1, H3, H4), while a longer alpha- helix (H2) forms the central core of the dimer interface [PMID: 15557262]. Some proteins known to contain a BFN domain are listed below:

  • Thermotoga maritima TM0160, a conserved hypothetical protein.
  • Plant bifunctional nuclease in basal defense response (BBD) proteins, regulators in abscisic acid (ABA)-mediated defense responses.

This entry represents the BFN domain.

GO terms

Biological Process

No terms assigned in this category.

Molecular Function

GO:0004518 nuclease activity

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE profiles