DNA-binding RFX-type winged-helix domain (IPR003150)

Short name: DNA-bd_RFX

Overlapping homologous superfamilies

Domain relationships



The RFX family of transcription factors is characterised by a unique approximately 75-residue DNA-binding domain. RFX genes have been isolated in yeasts, nematode and vertebrates. The characteristic RFX-type HTH DNA binding domain has been recruited into otherwise very divergent regulatory factors functioning in a diverse spectrum of unrelated systems, including regulation of the mitotic cell cycle in fission yeast, the control of the immune response in mammals,and infection by human hepatitis B virus [PMID: 8600444, PMID: 10767550].

The RFX-type DNA-binding domain is an unusual member of the winged-helix subfamily of helix-turn-helix domains because it uses a beta-hairpin (or wing) to recognise DNA instead of the recognition helix typical of helix-tun-helix domains. It consists of three alpha-helices (H), three beta-strands (S) and three connecting loops (L), arranged in the order H1-S1-H2-L1-H3-L2-S2-W1-S3. The third loop, connecting beta-strands S2 and S3, forms wing W1 of the winged-helix motif. In contrast to previously described winged-helix DBDs (typically 110 residues long with two wings, W1 and W2), the shorter RFX-type winged-helix DNA-binding domain has only one wing [PMID: 10706293].

GO terms

Biological Process

GO:0006355 regulation of transcription, DNA-templated

Molecular Function

GO:0003677 DNA binding

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE profiles