Glycoside hydrolase, family 27 (IPR002241)

Short name: Glyco_hydro_27

Overlapping homologous superfamilies

Family relationships


O-Glycosyl hydrolases (EC:3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [PMID: 7624375, PMID: 8535779]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site.

Glycoside hydrolase family 27 GH27 comprises enzymes with several known activities; alpha-galactosidase (EC:; alpha-N-acetylgalactosaminidase (EC:; isomalto-dextranase (EC:

Alpha-galactosidase (melibiase) catalyses the hydrolysis of melibiose into galactose and glucose [PMID: 7725791]. In man, deficiency in the enzyme results in Fabry's disease (X-linked sphingolipidosis). Alpha-N-acetylgalactosaminidase catalyses the hydrolysis of terminal non-reducing N-acetyl-D-galactosamine residues in N-acetyl-alpha-D-galactosaminides [PMID: 2174888]. Two conserved Asp residues may be involved in the catalytic mechanism in these enzymes. Deficiency in this enzyme results in Schindler and Kanzaki diseases.

GO terms

Biological Process

GO:0005975 carbohydrate metabolic process

Molecular Function

GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.