Glycoside hydrolase, family 24 (IPR002196)

Short name: Glyco_hydro_24

Overlapping homologous superfamilies

Family relationships


O-Glycosyl hydrolases (EC:3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [PMID: 7624375, PMID: 8535779]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site.

Glycoside hydrolase family 24 GH24 comprises enzymes with only one known activity; lysozyme (EC:

This entry includes Bacteriophage lambda lysozyme and Escherichia coli endolysin [PMID: 3586019]. Lysozyme helps to release mature phage particles from the cell wall by breaking down the peptidoglycan. The enzyme hydrolyses the 1,4-beta linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of prokaryotic cell walls. E. coli endolysin also functions in bacterial cell lysis and acts as a transglycosylase. The Bacteriophage T4 lysozyme structure contains two domains, the interface between which forms the active-site cleft. The N terminus of the two domains undergoes a 'hinge-bending' motion about an axis passing through the molecular waist [PMID: 3586019, PMID: 2234094]. This mobility is thought to be important in allowing access of substrates to the enzyme active site.

GO terms

Biological Process

GO:0016998 cell wall macromolecule catabolic process
GO:0009253 peptidoglycan catabolic process

Molecular Function

GO:0003796 lysozyme activity

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.