Barnase (IPR001887)

Short name: Barnase

Overlapping homologous superfamilies

Family relationships


Barnase is the extracellular ribonuclease of Bacillus amyloliquefaciens, and barstar its specific intracellular inhibitor [PMID: 2696173, PMID: 3050134]. Expression of barstar is necessary to counter the lethal effect of expressed active barnase. Barnase hydrolyses phosphodiester bonds in RNA, poly- and oligo-ribonucleotides, resulting in 3'-nucleoside monophosphates via 2',3'-cyclo-phospate intermediates. It is a small single-chain protein that has neither disulphide bonds nor non-peptide components. The NMR structure [PMID: 1888730] contains 2 alpha-helices and a 5-stranded anti-parallel beta-sheet. The protein core is formed by the packing of the first alpha-helix onto the beta-sheet.

GO terms

Biological Process

No terms assigned in this category.

Molecular Function

GO:0003723 RNA binding
GO:0004521 endoribonuclease activity

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.