-
Overview
-
Pathways & interactions
Barnase (IPR001887)
Short name: Barnase
Overlapping homologous superfamilies
- Ribonuclease/ribotoxin (IPR016191)
Family relationships
- Guanine-specific ribonuclease N1/T1/U2 (IPR000026)
- Barnase (IPR001887)
Description
Barnase is the extracellular ribonuclease of Bacillus amyloliquefaciens, and barstar its specific intracellular inhibitor [PMID: 2696173, PMID: 3050134]. Expression of barstar is necessary to counter the lethal effect of expressed active barnase. Barnase hydrolyses phosphodiester bonds in RNA, poly- and oligo-ribonucleotides, resulting in 3'-nucleoside monophosphates via 2',3'-cyclo-phospate intermediates. It is a small single-chain protein that has neither disulphide bonds nor non-peptide components. The NMR structure [PMID: 1888730] contains 2 alpha-helices and a 5-stranded anti-parallel beta-sheet. The protein core is formed by the packing of the first alpha-helix onto the beta-sheet.
GO terms
Biological Process
No terms assigned in this category.
Molecular Function
GO:0003723 RNA binding
GO:0004521 endoribonuclease activity
Cellular Component
No terms assigned in this category.
Contributing signatures
- PIRSF001013 (Barnase)
- PR00117 (BARNASE)
- cd00933 (barnase)