Active Site

Glycoside hydrolase, chitinase active site (IPR001579)

Short name: Glyco_hydro_18_chit_AS


O-Glycosyl hydrolases (EC:3.2.1.) are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [PMID: 7624375, PMID: 8535779]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site.

Chitinases (EC: [PMID: 1516675] are enzymes that catalyse the hydrolysis of the beta-1,4-N-acetyl-D-glucosamine linkages in chitin polymers. From the view point of sequence similarity chitinases belong to either family 18 or 19 in the classification of glycosyl hydrolases [PMID: 1747104].

Chitinases of family 18 (also known as classes III or V) groups a variety of chitinases and other proteins. Site directed mutagenesis experiments [PMID: 8103047] and crystallographic data [PMID: 7704527, PMID: 7704528] have shown that a conserved glutamate is involved in the catalytic mechanism and probably acts as a proton donor. This glutamate is the last residue of this active site signature.

GO terms

Biological Process

GO:0005975 carbohydrate metabolic process

Molecular Function

GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE patterns