tRNA-dihydrouridine synthase (IPR001269)

Short name: tRNA_hU_synthase

Overlapping homologous superfamilies

Family relationships


Members of this family catalyse the reduction of the 5,6-double bond of a uridine residue on tRNA. Dihydrouridine modification of tRNA is widely observed in prokaryotes and eukaryotes, and also in some archae. Most dihydrouridines are found in the D loop of t-RNAs. The role of dihydrouridine in tRNA is currently unknown, but may increase conformational flexibility of the tRNA. It is likely that different family members have different substrate specificities, which may overlap. Dus 1 (P53759) from Saccharomyces cerevisiae (Baker's yeast) acts on pre-tRNA-Phe, while Dus 2 (P53720) acts on pre-tRNA-Tyr and pre-tRNA-Leu. Dus 1 is active as a single subunit, requiring NADPH or NADH, and is stimulated by the presence of FAD [PMID: 12003496]. Some family members may be targeted to the mitochondria and even have a role in mitochondria [PMID: 12003496].

GO terms

Biological Process

GO:0055114 oxidation-reduction process
GO:0008033 tRNA processing

Molecular Function

GO:0050660 flavin adenine dinucleotide binding
GO:0017150 tRNA dihydrouridine synthase activity

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.