Conserved Site

Extradiol ring-cleavage dioxygenase, class I /II (IPR000486)

Short name: Xdiol_ring_cleave_dOase_1/2


Dioxygenases catalyse the incorporation of both atoms of molecular oxygen into substrates using a variety of reaction mechanisms. Cleavage of aromatic rings is one of the most important functions of dioxygenases, which play key roles in the degradation of aromatic compounds. The substrates of ring-cleavage dioxygenases can be classified into two groups according to the mode of scission of the aromatic ring. Intradiol enzymes (IPR000627) use a non-haem Fe(III) to cleave the aromatic ring between two hydroxyl groups (ortho-cleavage), whereas extradiol enzymes use a non-haem Fe(II) to cleave the aromatic ring between a hydroxylated carbon and an adjacent non-hydroxylated carbon (meta-cleavage) [PMID: 10730195, PMID: 15264822]. These two subfamilies differ in sequence, structural fold, iron ligands, and the orientation of second sphere active site amino acid residues. Extradiol dioxygenases are usually homo-multimeric, bind one atom of ferrous ion per subunit and have a subunit size of about 33 kDa. Extradiol dioxygenases can be divided into three classes. Class I and II enzymes show sequence similarity, with the two-domain class II enzymes having evolved from a class I enzyme through gene duplication. As a result, the class II enzymes are composed of two domains with approximately the same folding pattern as each other. Class III enzymes (IPR004183, IPR004183) are different in sequence and structure, but they do share several common active-site characteristics with the class II enzymes, in particular the coordination sphere and the disposition of the putative catalytic base are very similar. This entry represents the dioxygenase domain found in class I and class II extradiol enzymes.

Enzymes that belong to the extradiol class II family include catechol 2,3-dioxygenase (2,3-CTD) (EC: and biphenyl-2,3-diol 1,2-dioxygenase (BphC) (EC:

GO terms

Biological Process

GO:0006725 cellular aromatic compound metabolic process
GO:0055114 oxidation-reduction process

Molecular Function

GO:0003824 catalytic activity
GO:0008198 ferrous iron binding

Cellular Component

No terms assigned in this category.

Contributing signatures

Signatures from InterPro member databases are used to construct an entry.
PROSITE patterns