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InterPro: IPR018131 Restriction endonuclease, type II, EcoRI, Proteobacteria

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UniProtKB

Matches:

3 proteins

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Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
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Architectures
Accession List
Matches in BioMart

Accession

IPR018131 Restrict_endonuc_II_EcoRI_Pbac

Type

Family

Signatures Help

Database	ID	Name	Proteins
PIRSF	PIRSF001002	Restrict_endonuc_II_EcoRI	3
Signatures in BioMart

InterPro Relationships Help

Parent

IPR004221 Restriction endonuclease, type II, EcoRI

GO Term annotation Help

Process

GO:0009307 DNA restriction-modification system

Function

GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0009036 Type II site-specific deoxyribonuclease activity

InterPro annotation

Entry Details in BioMart

Abstract

This entry represents restriction endonucleases EcoRI in Proteobacteria, which requires magnesium as a cofactor. EcoRI recognises the DNA sequence GAATTC and cleaves after G-1 [1].

Structural links Help

PDB - click here

SCOP: c.52.1.1

CATH: 3.40.580.10

Database links Help

CluSTr: ALLvsALL:1228:70.7

PRIAM: PRI000339

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR018131

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

P00642 Type-2 restriction enzyme EcoRI

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR004221	Restriction endonuclease, type II, EcoRI
IPR011336	Restriction endonuclease, type II, EcoRI/MunI, core
IPR011335	Restriction endonuclease, type II-like, core
IPR018131	Restriction endonuclease, type II, EcoRI, Proteobacteria
	PDB Chain
	ModBase
	CATH Domain
	SCOP Domain

Publications Open in usermanual
1.	Watrob H, Liu W, Chen Y, Bartlett SG, Jen-Jacobson L, Barkley MD. Solution conformation of EcoRI restriction endonuclease changes upon binding of cognate DNA and Mg2+ cofactor. Biochemistry 40 683-92 2001 [PubMed: 11170385] http://dx.doi.org/10.1021/bi002055j

Additional Reading Open in usermanual
	Sapienza PJ, Rosenberg JM, Jen-Jacobson L. Structural and thermodynamic basis for enhanced DNA binding by a promiscuous mutant EcoRI endonuclease. Structure 15 2007 1368-82 [PubMed: 17997963] http://dx.doi.org/10.1016/j.str.2007.09.014
	Kim YC, Grable JC, Love R, Greene PJ, Rosenberg JM. Refinement of Eco RI endonuclease crystal structure: a revised protein chain tracing. Science 249 1990 1307-9 [PubMed: 2399465] http://www.sciencemag.org/cgi/content/abstract/249/4974/1307

InterPro 24.0