EBI Databases InterPro	Search Open in usermanual InterPro:
Jump to: InterProScan Databases Documentation FTP site Help Click on the icon for context sensitive help from the user manual. Advanced search

InterPro: IPR016135 Ubiquitin-conjugating enzyme/RWD-like

Protein matches Help

UniProtKB

Matches:

5342 proteins

Overview:	sorted by AC,	sorted by name,	of known structure,	proteins with splice variants
Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
Table:	For all matching proteins,	of known structure
Architectures
Accession List
Matches in BioMart

Accession

IPR016135 UBQ-conjugat/RWD-like

Type

Domain

Signatures Help

Database	ID	Name	Proteins
Gene3D	G3DSA:3.10.110.10	UBQ-conjugat_E2	4905
SuperFamily	SSF54495	UBQ-conjugat/RWD-like	5337
Signatures in BioMart

InterPro Relationships Help

Children

IPR000608 Ubiquitin-conjugating enzyme, E2
IPR006575 RWD

Found in

IPR014806 Ubiquitin-fold modifier-conjugating enzyme 1

InterPro annotation

Entry Details in BioMart

Abstract

This entry represents a structural domain with an alpha-beta(4)-alpha(3) core fold. Domains of this structure are found in:

Ubiquitin conjugating enzyme E2, as well as related proteins such as ubiquitin carrier protein 4 and ubiquitin-protein ligase W [1].
The UEV domain in tumour susceptibility gene 101 [2] and vacuolar protein sorting-associated protein [3].
RWD domain, found in RING finger and WD repeat-containing proteins, such as EIF-2 kinase 4 (GCN2-like protein) [4].
UFC1-like domain found in Ufm1-conjugating enzyme 1 [5].

Structural links Help

PDB - click here

SCOP: d.20.1.1 , d.20.1.2 , d.20.1.3

CATH: 3.10.110.10

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR016135

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

A1ZBR5 Protein crossbronx-like

O00762 Ubiquitin-conjugating enzyme E2 C

P06104 Ubiquitin-conjugating enzyme E2 2

P34477 Probable ubiquitin-conjugating enzyme E2 7

P60605 Ubiquitin-conjugating enzyme E2 G2

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR016135	Ubiquitin-conjugating enzyme/RWD-like
IPR000608	Ubiquitin-conjugating enzyme, E2
IPR015582	Ubiquitin-conjugating enzyme E2 H10
	PDB Chain
	ModBase
	CATH Domain
	SCOP Domain

Publications Open in usermanual
1.	Miura T, Klaus W, Ross A, Guntert P, Senn H. The NMR structure of the class I human ubiquitin-conjugating enzyme 2b. J. Biomol. NMR 22 89-92 2002 [PubMed: 11885984] http://dx.doi.org/10.1023/A:1013807519703
2.	Palencia A, Martinez JC, Mateo PL, Luque I, Camara-Artigas A. Structure of human TSG101 UEV domain. Acta Crystallogr. D Biol. Crystallogr. 62 458-64 2006 [PubMed: 16552148] http://dx.doi.org/10.1107/S0907444906005221
3.	Teo H, Veprintsev DB, Williams RL. Structural insights into endosomal sorting complex required for transport (ESCRT-I) recognition of ubiquitinated proteins. J. Biol. Chem. 279 28689-96 2004 [PubMed: 15044434] http://dx.doi.org/10.1074/jbc.M400023200
4.	Nameki N, Yoneyama M, Koshiba S, Tochio N, Inoue M, Seki E, Matsuda T, Tomo Y, Harada T, Saito K, Kobayashi N, Yabuki T, Aoki M, Nunokawa E, Matsuda N, Sakagami N, Terada T, Shirouzu M, Yoshida M, Hirota H, Osanai T, Tanaka A, Arakawa T, Carninci P, Kawai J, Hayashizaki Y, Kinoshita K, Guntert P, Kigawa T, Yokoyama S. Solution structure of the RWD domain of the mouse GCN2 protein. Protein Sci. 13 2089-100 2004 [PubMed: 15273307] http://dx.doi.org/10.1110/ps.04751804
5.	Mizushima T, Tatsumi K, Ozaki Y, Kawakami T, Suzuki A, Ogasahara K, Komatsu M, Kominami E, Tanaka K, Yamane T. Crystal structure of Ufc1, the Ufm1-conjugating enzyme. Biochem. Biophys. Res. Commun. 362 1079-84 2007 [PubMed: 17825256] http://dx.doi.org/10.1016/j.bbrc.2007.08.129

Additional Reading Open in usermanual
	Mace PD, Linke K, Feltham R, Schumacher FR, Smith CA, Vaux DL, Silke J, Day CL. Structures of the cIAP2 RING domain reveal conformational changes associated with ubiquitin-conjugating enzyme (E2) recruitment. J. Biol. Chem. 283 2008 31633-40 [PubMed: 18784070] http://dx.doi.org/10.1074/jbc.M804753200
	Wang J, Hu W, Cai S, Lee B, Song J, Chen Y. The intrinsic affinity between E2 and the Cys domain of E1 in ubiquitin-like modifications. Mol. Cell 27 2007 228-37 [PubMed: 17643372] http://dx.doi.org/10.1016/j.molcel.2007.05.023
	Vedadi M, Lew J, Artz J, Amani M, Zhao Y, Dong A, Wasney GA, Gao M, Hills T, Brokx S, Qiu W, Sharma S, Diassiti A, Alam Z, Melone M, Mulichak A, Wernimont A, Bray J, Loppnau P, Plotnikova O, Newberry K, Sundararajan E, Houston S, Walker J, Tempel W, Bochkarev A, Kozieradzki I, Edwards A, Arrowsmith C, Roos D, Kain K, Hui R. Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms. Mol. Biochem. Parasitol. 151 2007 100-10 [PubMed: 17125854] http://dx.doi.org/10.1016/j.molbiopara.2006.10.011
	Wilson RC, Hughes RC, Flatt JW, Meehan EJ, Ng JD, Twigg PD. Structure of full-length ubiquitin-conjugating enzyme E2-25K (huntingtin-interacting protein 2). Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 65 2009 440-4 [PubMed: 19407372]
	Knipscheer P, Flotho A, Klug H, Olsen JV, van Dijk WJ, Fish A, Johnson ES, Mann M, Sixma TK, Pichler A. Ubc9 sumoylation regulates SUMO target discrimination. Mol. Cell 31 2008 371-82 [PubMed: 18691969] http://dx.doi.org/10.1016/j.molcel.2008.05.022

InterPro 23.1