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InterPro: IPR015827 Alpha-(1,6)-fucosyltransferase, eukaryotic type
Protein matches
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UniProtKB Matches: 40 proteins |
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Accession
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IPR015827 Alpha1_6FUT_euk |
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Type
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Family |
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Signatures
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InterPro Relationships
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Contains
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IPR001452 Src homology-3 domain
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GO Term annotation
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Process
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GO:0033578 protein amino acid glycosylation in Golgi
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Function
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GO:0008424 glycoprotein 6-alpha-L-fucosyltransferase activity
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Component
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GO:0016021 integral to membrane
GO:0032580 Golgi cisterna membrane
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InterPro annotation
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 Entry Details in BioMart
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Abstract
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Members of this group have enzymatic activity EC:2.4.1.68. They transfer fucose on glycoproteins to the innermost asparagine-linked N-acetylglucosamine of the chitobiose disaccharide-core unit [1]. Core alpha 1,6-fucosylation is a conserved feature of animal N-linked oligosaccharides, being present in both invertebrates and vertebrates [2]. All known fucosyltransferases are type II transmembrane proteins with four distinct domains: cytoplasmic, transmembrane, hypervariable, and catalytic. The catalytic domain resides in the luminal side of the trans-Golgi compartment [3]. Members of this group share the conserved three alpha-6-motifs that are also present in the bacterial 6-alpha fucosyltransferases (IPR008716) [4]. Disruption of the 6-alpha fucosyltransferase gene from a Chinese hamster ovary cell line has been shown to produce completely defucosylated recombinant antibodies [5]. All members contain an SH3 domain (IPR001452) near the carboxyl end, but its function is unknown.
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Database links
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Publications
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1.
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Oriol R, Mollicone R, Cailleau A, Balanzino L, Breton C.
Divergent evolution of fucosyltransferase genes from vertebrates, invertebrates, and bacteria.
Glycobiology 9 323-34 1999
[PubMed: 10089206]
http://dx.doi.org/10.1093/glycob/9.4.323
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2.
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Paschinger K, Staudacher E, Stemmer U, Fabini G, Wilson IB.
Fucosyltransferase substrate specificity and the order of fucosylation in invertebrates.
Glycobiology 15 463-74 2005
[PubMed: 15604090]
http://dx.doi.org/10.1093/glycob/cwi028
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3.
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Paulson JC, Colley KJ.
Glycosyltransferases. Structure, localization, and control of cell type-specific glycosylation.
J. Biol. Chem. 264 17615-8 1989
[PubMed: 2681181]
http://intl.jbc.org/cgi/content/abstract/264/30/17615
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4.
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Breton C, Oriol R, Imberty A.
Conserved structural features in eukaryotic and prokaryotic fucosyltransferases.
Glycobiology 8 87-94 1998
[PubMed: 9451017]
http://dx.doi.org/10.1093/glycob/8.1.87
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5.
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Yamane-Ohnuki N, Kinoshita S, Inoue-Urakubo M, Kusunoki M, Iida S, Nakano R, Wakitani M, Niwa R, Sakurada M, Uchida K, Shitara K, Satoh M.
Establishment of FUT8 knockout Chinese hamster ovary cells: an ideal host cell line for producing completely defucosylated antibodies with enhanced antibody-dependent cellular cytotoxicity.
Biotechnol. Bioeng. 87 614-22 2004
[PubMed: 15352059]
http://dx.doi.org/10.1002/bit.20151
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InterPro 23.1
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