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InterPro: IPR012328 Chalcone/stilbene synthase, C-terminal

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2177 proteins

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Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
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Accession List
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Accession

IPR012328 Chalcone/stilbene_synth_C

Secondary

IPR001099

Type

Domain

Signatures Help

Database	ID	Name	Proteins
Pfam	PF02797	Chal_sti_synt_C	2177
Signatures in BioMart

InterPro Relationships Help

Found in

IPR011141 Polyketide synthase, type III
IPR012392 Very-long-chain 3-ketoacyl-CoA synthase
IPR016038 Thiolase-like, subgroup
IPR016039 Thiolase-like

GO Term annotation Help

Function

GO:0008415 acyltransferase activity

InterPro annotation

Entry Details in BioMart

Abstract

Synonym(s): Chalcone synthase, Flavonone synthase, 6'-deoxychalcone synthase

Naringenin-chalcone synthases (EC:2.3.1.74) and stilbene synthases (STS) (formerly known as resveratrol synthases) are related plant enzymes. CHS is an important enzyme in flavanoid biosynthesis and STS is a key enzyme in stilbene-type phyloalexin biosynthesis. Both enzymes catalyze the addition of three molecules of malonyl-CoA to a starter CoA ester (a typical example is 4-coumaroyl-CoA), producing either a chalcone (with CHS) or stilbene (with STS) [1].

These enzymes have a conserved cysteine residue, located in the central section of the protein sequence, which is essential for the catalytic activity of both enzymes and probably represents the binding site for the 4-coumaryl-CoA group [2].

This domain of chalcone synthase is reported to be structurally similar to domains in thiolase and beta-ketoacyl synthase. The differences in activity are accounted for by differences in the N-terminal domain.

Structural links Help

PDB - click here

SCOP: c.95.1.2

CATH: 3.40.47.10

Database links Help

Enzyme: EC:2.3.1

PANDIT: PF02797

Pfam Clan: CL0046.12

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR012328

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

A2ZEX7 Chalcone synthase

O48780 3-ketoacyl-CoA synthase 11

P30074 Chalcone synthase 2

P54157 Putative chalcone synthase

Q55E72 Probable polyketide synthase 1

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR001227	Acyl transferase domain
IPR020843	Polyketide synthase, enoylreductase
IPR016039	Thiolase-like
IPR016038	Thiolase-like, subgroup
IPR014030	Beta-ketoacyl synthase, N-terminal
IPR018201	Beta-ketoacyl synthase, active site
IPR016036	Malonyl-CoA ACP transacylase, ACP-binding
IPR020841	Polyketide synthase, beta-ketoacyl synthase region
IPR014031	Beta-ketoacyl synthase, C-terminal
IPR016035	Acyl transferase/acyl hydrolase/lysophospholipase
IPR020801	Polyketide synthase, acyl transferase domain
IPR011141	Polyketide synthase, type III
IPR013601	FAE1/Type III polyketide synthase-like protein
IPR011032	GroES-like
IPR016040	NAD(P)-binding domain
IPR009081	Acyl carrier protein-like
IPR000794	Beta-ketoacyl synthase
IPR012392	Very-long-chain 3-ketoacyl-CoA synthase
IPR013154	Alcohol dehydrogenase GroES-like
IPR001099	Chalcone/stilbene synthase, N-terminal
IPR014043	Acyl transferase
IPR012328	Chalcone/stilbene synthase, C-terminal
IPR018088	Chalcone/stilbene synthase, active site
IPR013968	Polyketide synthase, KR
IPR013217	Methyltransferase type 12
	ModBase
	SWISS-MODEL
	PDB Chain
	CATH Domain
	SCOP Domain

Publications Open in usermanual
1.	Schroeder J, Schroeder G. Stilbene and chalcone synthases: related enzymes with key functions in plant-specific pathways. Z. Naturforsch. C 45 1-8 1990
2.	Lanz T, Tropf S, Marner FJ, Schroder J, Schroder G. The role of cysteines in polyketide synthases. Site-directed mutagenesis of resveratrol and chalcone synthases, two key enzymes in different plant-specific pathways. J. Biol. Chem. 266 9971-6 1991 [PubMed: 2033084] http://intl.jbc.org/cgi/content/abstract/266/15/9971

Additional Reading Open in usermanual
	Austin MB, Izumikawa M, Bowman ME, Udwary DW, Ferrer JL, Moore BS, Noel JP. Crystal structure of a bacterial type III polyketide synthase and enzymatic control of reactive polyketide intermediates. J. Biol. Chem. 279 2004 45162-74 [PubMed: 15265863] http://dx.doi.org/10.1074/jbc.M406567200
	Ferrer JL, Jez JM, Bowman ME, Dixon RA, Noel JP. Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis. Nat. Struct. Biol. 6 1999 775-84 [PubMed: 10426957] http://dx.doi.org/10.1038/11553
	Sankaranarayanan R, Saxena P, Marathe UB, Gokhale RS, Shanmugam VM, Rukmini R. A novel tunnel in mycobacterial type III polyketide synthase reveals the structural basis for generating diverse metabolites. Nat. Struct. Mol. Biol. 11 2004 894-900 [PubMed: 15286723] http://dx.doi.org/10.1038/nsmb809
	Shomura Y, Torayama I, Suh DY, Xiang T, Kita A, Sankawa U, Miki K. Crystal structure of stilbene synthase from Arachis hypogaea. Proteins 60 2005 803-6 [PubMed: 16028220] http://dx.doi.org/10.1002/prot.20584
	Rukmini R, Shanmugam VM, Saxena P, Gokhale RS, Sankaranarayanan R. Crystallization and preliminary X-ray crystallographic investigations of an unusual type III polyketide synthase PKS18 from Mycobacterium tuberculosis. Acta Crystallogr. D Biol. Crystallogr. 60 2004 749-51 [PubMed: 15039574] http://dx.doi.org/10.1107/S0907444904002367
	Austin MB, Bowman ME, Ferrer JL, Schroder J, Noel JP. An aldol switch discovered in stilbene synthases mediates cyclization specificity of type III polyketide synthases. Chem. Biol. 11 2004 1179-94 [PubMed: 15380179] http://dx.doi.org/10.1016/j.chembiol.2004.05.024

InterPro 23.1