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InterPro: IPR008278 4'-phosphopantetheinyl transferase

Protein matches Help

UniProtKB

Matches:

3164 proteins

Overview:	sorted by AC,	sorted by name,	of known structure,	proteins with splice variants
Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
Table:	For all matching proteins,	of known structure
Architectures
Accession List
Matches in BioMart

Accession

IPR008278 4-PPantetheinyl_Trfase

Secondary

IPR002582 , IPR004568

Type

Domain

Signatures Help

Database	ID	Name	Proteins
Pfam	PF01648	ACPS	2991
SuperFamily	SSF56214	4-PPT_transf	3151
Signatures in BioMart

InterPro Relationships Help

Children

IPR004568 Phosphopantethiene-protein transferase
IPR016614 Holo-[acyl-carrier-protein] synthase, fungi

Found in

IPR003542 Enterobactin synthetase-like, component D

GO Term annotation Help

Process

GO:0009059 macromolecule biosynthetic process

Function

GO:0000287 magnesium ion binding
GO:0008897 holo-[acyl-carrier-protein] synthase activity

InterPro annotation

Entry Details in BioMart

Abstract

These proteins transfer the 4'-phosphopantetheine (4'-PP) moiety from coenzyme A (CoA) to the invariant serine of pp-binding. This post-translational modification renders holo-ACP capable of acyl group activation via thioesterification of the cysteamine thiol of 4'-PP [1]. This superfamily consists of two subtypes: The ACPS type such as ACPS_ECOLI and the Sfp type such as SFP_BACSU. The structure of the Sfp type is known [2], which shows the active site accommodates a magnesium ion. The most highly conserved regions of the alignment are involved in binding the magnesium ion.

Structural links Help

PDB - click here

SCOP: d.150.1.1 , d.150.1.2

CATH: 3.90.470.20

Database links Help

Enzyme: EC:2.7.8.7

PANDIT: PF01648

Blocks: IPB008278

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR008278

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

O86785 Holo-[acyl-carrier-protein] synthase

P19097 Fatty acid synthase subunit alpha

Q55185 Putative 4'-phosphopantetheinyl transferase slr0495

Q9CQF6 L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase

Q9NRN7 L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR001227	Acyl transferase domain
IPR016039	Thiolase-like
IPR014030	Beta-ketoacyl synthase, N-terminal
IPR016038	Thiolase-like, subgroup
IPR018201	Beta-ketoacyl synthase, active site
IPR014031	Beta-ketoacyl synthase, C-terminal
IPR008278	4'-phosphopantetheinyl transferase
IPR016035	Acyl transferase/acyl hydrolase/lysophospholipase
IPR004568	Phosphopantethiene-protein transferase
IPR016040	NAD(P)-binding domain
IPR000794	Beta-ketoacyl synthase
IPR002582	Holo-[acyl carrier protein] synthase
	PDB Chain
	ModBase
	CATH Domain
	SWISS-MODEL

Publications Open in usermanual
1.	Lambalot RH, Walsh CT. Cloning, overproduction, and characterization of the Escherichia coli holo-acyl carrier protein synthase. J. Biol. Chem. 270 24658-61 1995 [PubMed: 7559576] http://dx.doi.org/10.1074/jbc.270.42.24658
2.	Reuter K, Mofid MR, Marahiel MA, Ficner R. Crystal structure of the surfactin synthetase-activating enzyme sfp: a prototype of the 4'-phosphopantetheinyl transferase superfamily. EMBO J. 18 6823-31 1999 [PubMed: 10581256] http://dx.doi.org/10.1093/emboj/18.23.6823

Additional Reading Open in usermanual
	Parris KD, Lin L, Tam A, Mathew R, Hixon J, Stahl M, Fritz CC, Seehra J, Somers WS. Crystal structures of substrate binding to Bacillus subtilis holo-(acyl carrier protein) synthase reveal a novel trimeric arrangement of molecules resulting in three active sites. Structure 8 2000 883-95 [PubMed: 10997907] http://dx.doi.org/10.1016/S0969-2126(00)00178-7
	Chirgadze NY, Briggs SL, McAllister KA, Fischl AS, Zhao G. Crystal structure of Streptococcus pneumoniae acyl carrier protein synthase: an essential enzyme in bacterial fatty acid biosynthesis. EMBO J. 19 2000 5281-7 [PubMed: 11032795] http://dx.doi.org/10.1093/emboj/19.20.5281
	Lambalot RH, Gehring AM, Flugel RS, Zuber P, LaCelle M, Marahiel MA, Reid R, Khosla C, Walsh CT. A new enzyme superfamily - the phosphopantetheinyl transferases. Chem. Biol. 3 1996 923-36 [PubMed: 8939709] http://dx.doi.org/10.1016/S1074-5521(96)90181-7

InterPro 23.1