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InterPro: IPR007197 Radical SAM

Protein matches Help

UniProtKB

Matches:

28359 proteins

Overview:	sorted by AC,	sorted by name,	of known structure,	proteins with splice variants
Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
Table:	For all matching proteins,	of known structure
Architectures
Accession List
Matches in BioMart

Accession

IPR007197 Radical_SAM

Type

Domain

Signatures Help

Database	ID	Name	Proteins
Pfam	PF04055	Radical_SAM	28359
Signatures in BioMart

InterPro Relationships Help

Children

IPR006638 Elongator protein 3/MiaB/NifB

Found in

IPR003739 Protein of unknown function DUF160
IPR004383 Ribosomal RNA large subunit methyltransferase RlmN;
IPR011352 Pyruvate formate-lyase 2 activating enzyme
IPR012726 Thiazole biosynthesis ThiH
IPR012837 Ribonucleoside-triphosphate reductase activating, anaerobic
IPR012838 Pyruvate formate-lyase activating
IPR012839 Glycyl radical enzyme-activating
IPR012840 Ribonucleoside-triphosphate reductase, anaerobic-like
IPR016771 Fe-S oxidoreductase, radical SAM domain-containing, TM0948, predicted
IPR016863 Reductase, EryCV, predicted
IPR017742 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE
IPR019939 FO synthase, subunit 1
IPR019940 Complex F420, CofH
IPR020050 FO synthase, subunit 2

Contains

IPR000385 MoaA/nifB/pqqE, iron-sulphur binding, conserved site
IPR001989 Radical-activating enzyme, conserved site
IPR020612 Methylthiotransferase, conserved site

GO Term annotation Help

Function

GO:0003824 catalytic activity
GO:0051536 iron-sulfur cluster binding

InterPro annotation

Entry Details in BioMart

Abstract

Radical SAM proteins catalyze diverse reactions, including unusual methylations, isomerization, sulphur insertion, ring formation, anaerobic oxidation and protein radical formation. Evidence exists that these proteins generate a radical species by reductive cleavage of S:-adenosylmethionine (SAM) through an unusual Fe-S centre [1, 2].

Structural links Help

PDB - click here

SCOP: c.1.28.1 , c.1.28.2 , c.1.28.3

CATH: 3.20.20.70

Database links Help

Enzyme: EC:2

PANDIT: PF04055

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR007197

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

O43766 Lipoyl synthase, mitochondrial

P32451 Biotin synthase, mitochondrial

Q09316 CDK5RAP1-like protein

Q5RKZ7 Molybdenum cofactor biosynthesis protein 1

Q7K4W1 CDKAL1-like protein

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR010505	Molybdenum cofactor synthesis C-terminal
IPR013785	Aldolase-type TIM barrel
IPR002684	Biotin synthase
IPR010722	Biotin/thiamin synthesis-associated protein
IPR013848	Methylthiotransferase, N-terminal
IPR006466	MiaB-like tRNA modifying enzyme, archaeal-type
IPR005839	Methylthiotransferase
IPR000385	MoaA/nifB/pqqE, iron-sulphur binding, conserved site
IPR003698	Lipoate synthase
IPR013483	Molybdenum cofactor biosynthesis protein A
IPR002792	Deoxyribonuclease/rho motif-related TRAM
IPR007197	Radical SAM
IPR020612	Methylthiotransferase, conserved site
IPR006638	Elongator protein 3/MiaB/NifB
IPR002820	Molybdopterin cofactor biosynthesis C (MoaC) domain
	ModBase
	SWISS-MODEL

Publications Open in usermanual
1.	Sofia HJ, Chen G, Hetzler BG, Reyes-Spindola JF, Miller NE. Radical SAM, a novel protein superfamily linking unresolved steps in familiar biosynthetic pathways with radical mechanisms: functional characterization using new analysis and information visualization methods. Nucleic Acids Res. 29 1097-106 2001 [PubMed: 11222759] http://dx.doi.org/10.1093/nar/29.5.1097
2.	Hanzelmann P, Schindelin H. Crystal structure of the S-adenosylmethionine-dependent enzyme MoaA and its implications for molybdenum cofactor deficiency in humans. Proc. Natl. Acad. Sci. U.S.A. 101 12870-5 2004 [PubMed: 15317939] http://dx.doi.org/10.1073/pnas.0404624101

Additional Reading Open in usermanual
	Berkovitch F, Nicolet Y, Wan JT, Jarrett JT, Drennan CL. Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme. Science 303 2004 76-9 [PubMed: 14704425] http://dx.doi.org/10.1126/science.1088493
	Layer G, Moser J, Heinz DW, Jahn D, Schubert WD. Crystal structure of coproporphyrinogen III oxidase reveals cofactor geometry of Radical SAM enzymes. EMBO J. 22 2003 6214-24 [PubMed: 14633981] http://dx.doi.org/10.1093/emboj/cdg598
	Berteau O, Guillot A, Benjdia A, Rabot S. A new type of bacterial sulfatase reveals a novel maturation pathway in prokaryotes. J. Biol. Chem. 281 2006 22464-70 [PubMed: 16766528] http://dx.doi.org/10.1074/jbc.M602504200
	Benjdia A, Leprince J, Guillot A, Vaudry H, Rabot S, Berteau O. Anaerobic sulfatase-maturating enzymes: radical SAM enzymes able to catalyze in vitro sulfatase post-translational modification. J. Am. Chem. Soc. 129 2007 3462-3 [PubMed: 17335281] http://dx.doi.org/10.1021/ja067175e
	Hanzelmann P, Schindelin H. Binding of 5'-GTP to the C-terminal FeS cluster of the radical S-adenosylmethionine enzyme MoaA provides insights into its mechanism. Proc. Natl. Acad. Sci. U.S.A. 103 2006 6829-34 [PubMed: 16632608] http://dx.doi.org/10.1073/pnas.0510711103

InterPro 23.1