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InterPro: IPR005027 Glycosyl transferase, family 43

Protein matchesHelp
UniProtKB
Matches:
323 proteins
AccessionHelp IPR005027 Glyco_trans_43
TypeHelp Family
SignaturesHelp
GO Term annotationHelp
Function GO:0015018 galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity
Component GO:0016020 membrane
InterPro annotation
BioMart Logo Entry Details in BioMart
AbstractHelp

The biosynthesis of disaccharides, oligosaccharides and polysaccharides involves the action of hundreds of different glycosyltransferases. These enzymes catalyse the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. A classification of glycosyltransferases using nucleotide diphospho-sugar, nucleotide monophospho-sugar and sugar phosphates (EC:2.4.1.-) and related proteins into distinct sequence based families has been described [1]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site [2]. The same three-dimensional fold is expected to occur within each of the families. Because 3-D structures are better conserved than sequences, several of the families defined on the basis of sequence similarities may have similar 3-D structures and therefore form 'clans'.

Glycosyltransferase family 43 GT43 comprises enzymes with only one known activities; beta-glucuronyltransferase(EC:2.4.1);.

Structural linksHelp
SCOP: c.68.1.7
CATH: 3.90.550.10
Database linksHelp
Enzyme: EC:2.4.1.135
CAZy: GT43
PANDIT: PF03360
Blocks: IPB005027
Pfam Clan: CL0110.8

Taxonomic coverageHelp

Example proteinsHelp
O35789 Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1

O94766 Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3

O97422 Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase I

P58158 Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3

Q09363 Probable glucuronosyltransferase sqv-8

More proteins


Example Proteins Key


InterPro entry accession number/name and structure databases Colour code
IPR005027 Glycosyl transferase, family 43
SWISS-MODEL
PDB Chain
ModBase
SCOP Domain
CATH Domain

PublicationsHelp
1. Campbell JA, Davies GJ, Bulone V, Henrissat B.
A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities.
Biochem. J. 326 ( Pt 3) 929-39 1997 [PubMed: 9334165]
http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=9334165
2. Henrissat B, Coutinho PM.
Carbohydrate-Active Enzymes server.
1999

Additional ReadingHelp
Kakuda S, Shiba T, Ishiguro M, Tagawa H, Oka S, Kajihara Y, Kawasaki T, Wakatsuki S, Kato R.
Structural basis for acceptor substrate recognition of a human glucuronyltransferase, GlcAT-P, an enzyme critical in the biosynthesis of the carbohydrate epitope HNK-1.
J. Biol. Chem. 279 2004 22693-703 [PubMed: 14993226]
http://dx.doi.org/10.1074/jbc.M400622200
Tone Y, Pedersen LC, Yamamoto T, Izumikawa T, Kitagawa H, Nishihara J, Tamura J, Negishi M, Sugahara K.
2-o-phosphorylation of xylose and 6-o-sulfation of galactose in the protein linkage region of glycosaminoglycans influence the glucuronyltransferase-I activity involved in the linkage region synthesis.
J. Biol. Chem. 283 2008 16801-7 [PubMed: 18400750]
http://dx.doi.org/10.1074/jbc.M709556200
Pedersen LC, Tsuchida K, Kitagawa H, Sugahara K, Darden TA, Negishi M.
Heparan/chondroitin sulfate biosynthesis. Structure and mechanism of human glucuronyltransferase I.
J. Biol. Chem. 275 2000 34580-5 [PubMed: 10946001]
http://dx.doi.org/10.1074/jbc.M007399200
Terayama K, Seiki T, Nakamura A, Matsumori K, Ohta S, Oka S, Sugita M, Kawasaki T.
Purification and characterization of a glucuronyltransferase involved in the biosynthesis of the HNK-1 epitope on glycoproteins from rat brain.
J. Biol. Chem. 273 1998 30295-300 [PubMed: 9804790]
http://dx.doi.org/10.1074/jbc.273.46.30295
Pedersen LC, Darden TA, Negishi M.
Crystal structure of beta 1,3-glucuronyltransferase I in complex with active donor substrate UDP-GlcUA.
J. Biol. Chem. 277 2002 21869-73 [PubMed: 11950836]
http://dx.doi.org/10.1074/jbc.M112343200
Wei G, Bai X, Sarkar AK, Esko JD.
Formation of HNK-1 determinants and the glycosaminoglycan tetrasaccharide linkage region by UDP-GlcUA:Galactose beta1, 3-glucuronosyltransferases.
J. Biol. Chem. 274 1999 7857-64 [PubMed: 10075678]
http://dx.doi.org/10.1074/jbc.274.12.7857
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InterPro 23.1