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InterPro: IPR004607 Phosphoribosylglycinamide formyltransferase

Protein matches Help

UniProtKB

Matches:

1633 proteins

Overview:	sorted by AC,	sorted by name,	of known structure,	proteins with splice variants
Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
Table:	For all matching proteins,	of known structure
Architectures
Accession List
Matches in BioMart

Accession

IPR004607 PurN_trans

Type

Family

Signatures Help

Database	ID	Name	Proteins
TIGRFAMs	TIGR00639	PurN	1633
Signatures in BioMart

InterPro Relationships Help

Contains

IPR001555 Phosphoribosylglycinamide formyltransferase, active site
IPR002376 Formyl transferase, N-terminal

GO Term annotation Help

Process

GO:0006189 'de novo' IMP biosynthetic process

Function

GO:0004644 phosphoribosylglycinamide formyltransferase activity

InterPro annotation

Entry Details in BioMart

Abstract

This family describes phosphoribosylglycinamide formyltransferase (GAR transformylase), one of several proteins found in formyltransferase. This enzyme uses formyl tetrahydrofolate as a formyl group donor to produce 5'-phosphoribosyl-N-formylglycinamide. PurT, a different GAR transformylase, uses ATP and formate rather than formyl tetrahydrofolate. Experimental proof includes complementation of Escherichia coli purN mutants by orthologs from vertebrates (where it is a domain of a multifunctional protein), Bacillus subtilis, and Arabidopsis thaliana. In phylogenetic analyses, the member from Saccharomyces cerevisiae shows a long branch length but membership in the family, while the formyltetrahydrofolate deformylases form a closely related outgroup.

Structural links Help

PDB - click here

SCOP: c.65.1.1

CATH: 3.40.50.170

Database links Help

Enzyme: EC:2.1.2.2

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR004607

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

P00967 Trifunctional purine biosynthetic protein adenosine-3

P04161 Phosphoribosylglycinamide formyltransferase

P22102 Trifunctional purine biosynthetic protein adenosine-3

P52422 Phosphoribosylglycinamide formyltransferase, chloroplastic

Q64737 Trifunctional purine biosynthetic protein adenosine-3

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR013815	ATP-grasp fold, subdomain 1
IPR013816	ATP-grasp fold, subdomain 2
IPR002376	Formyl transferase, N-terminal
IPR013817	Pre-ATP-grasp fold
IPR020559	Phosphoribosylglycinamide synthetase, conserved site
IPR004607	Phosphoribosylglycinamide formyltransferase
IPR016188	PurM, N-terminal-like
IPR016185	PreATP-grasp-like fold
IPR011761	ATP-grasp fold
IPR001555	Phosphoribosylglycinamide formyltransferase, active site
IPR010918	AIR synthase related protein, C-terminal
IPR004733	Phosphoribosylformylglycinamidine cyclo-ligase
IPR011054	Rudiment single hybrid motif
IPR020561	Phosphoribosylglycinamide synthetase, ATP-grasp (A) domain
IPR020560	Phosphoribosylglycinamide synthetase, C-domain
IPR020562	Phosphoribosylglycinamide synthetase, N-domain
IPR000728	AIR synthase related protein
IPR000115	Phosphoribosylglycinamide synthetase
	PDB Chain
	ModBase
	CATH Domain
	SWISS-MODEL
	SCOP Domain

Publications Help

Additional Reading Open in usermanual
	Dahms TE, Sainz G, Giroux EL, Caperelli CA, Smith JL. The apo and ternary complex structures of a chemotherapeutic target: human glycinamide ribonucleotide transformylase. Biochemistry 44 2005 9841-50 [PubMed: 16026156] http://dx.doi.org/10.1021/bi050307g
	Greasley SE, Marsilje TH, Cai H, Baker S, Benkovic SJ, Boger DL, Wilson IA. Unexpected formation of an epoxide-derived multisubstrate adduct inhibitor on the active site of GAR transformylase. Biochemistry 40 2001 13538-47 [PubMed: 11695901] http://dx.doi.org/10.1021/bi011482+
	Smith JM, Daum HA 3rd. Identification and nucleotide sequence of a gene encoding 5'-phosphoribosylglycinamide transformylase in Escherichia coli K12. J. Biol. Chem. 262 1987 10565-9 [PubMed: 3301838] http://intl.jbc.org/cgi/reprint/262/22/10565.pdf
	Zhang Y, Desharnais J, Greasley SE, Beardsley GP, Boger DL, Wilson IA. Crystal structures of human GAR Tfase at low and high pH and with substrate beta-GAR. Biochemistry 41 2002 14206-15 [PubMed: 12450384] http://dx.doi.org/10.1021/bi020522m
	Zhang Y, Desharnais J, Marsilje TH, Li C, Hedrick MP, Gooljarsingh LT, Tavassoli A, Benkovic SJ, Olson AJ, Boger DL, Wilson IA. Rational design, synthesis, evaluation, and crystal structure of a potent inhibitor of human GAR Tfase: 10-(trifluoroacetyl)-5,10-dideazaacyclic-5,6,7,8-tetrahydrofolic acid. Biochemistry 42 2003 6043-56 [PubMed: 12755606] http://dx.doi.org/10.1021/bi034219c
	Greasley SE, Yamashita MM, Cai H, Benkovic SJ, Boger DL, Wilson IA. New insights into inhibitor design from the crystal structure and NMR studies of Escherichia coli GAR transformylase in complex with beta-GAR and 10-formyl-5,8,10-trideazafolic acid. Biochemistry 38 1999 16783-93 [PubMed: 10606510] http://dx.doi.org/10.1021/bi991888a

InterPro 23.1