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InterPro: IPR004276 Glycosyl transferase, family 28

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UniProtKB

Matches:

2524 proteins

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Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
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Accession

IPR004276 Glyco_trans_28

Type

Domain

Signatures Help

Database	ID	Name	Proteins
Pfam	PF03033	Glyco_transf_28	2524
Signatures in BioMart

InterPro Relationships Help

Found in

IPR006009 N-acetylglucosaminyltransferase, MurG

GO Term annotation Help

Process

GO:0005975 carbohydrate metabolic process
GO:0030259 lipid glycosylation

Function

GO:0016758 transferase activity, transferring hexosyl groups

InterPro annotation

Entry Details in BioMart

Abstract

The biosynthesis of disaccharides, oligosaccharides and polysaccharides involves the action of hundreds of different glycosyltransferases. These enzymes catalyse the transfer of sugar moieties from activated donor molecules to specific acceptor molecules, forming glycosidic bonds. A classification of glycosyltransferases using nucleotide diphospho-sugar, nucleotide monophospho-sugar and sugar phosphates (EC:2.4.1.-) and related proteins into distinct sequence based families has been described [1]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site [2]. The same three-dimensional fold is expected to occur within each of the families. Because 3-D structures are better conserved than sequences, several of the families defined on the basis of sequence similarities may have similar 3-D structures and therefore form 'clans'.

Glycosyltransferase family 28 GT28 comprises enzymes with a number of known activities; 1,2-diacylglycerol 3-beta-galactosyltransferase (EC:2.4.1.46); 1,2-diacylglycerol 3-beta-glucosyltransferase (EC:2.4.1.157); beta-N-acetylglucosamine transferase (EC:2.4.1).

Structural links Help

PDB - click here

SCOP: c.87.1.2 , c.87.1.5

CATH: 3.40.50.2000

Database links Help

Enzyme: EC:2.4.1.227

CAZy: GT28

PANDIT: PF03033

Blocks: IPB004276

Pfam Clan: CL0113.9

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR004276

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

A1CFB3 Sterol 3-beta-glucosyltransferase

A7A179 Sterol 3-beta-glucosyltransferase

P17443 UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase

P74657 UDP-N-acetylglucosamine--N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase

Q8PWF3 Uncharacterized glycosyltransferase MM_1636

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR011993	Pleckstrin homology-type
IPR002213	UDP-glucuronosyl/UDP-glucosyltransferase
IPR007235	Glycosyl transferase, family 28, C-terminal
IPR001849	Pleckstrin homology
IPR006009	N-acetylglucosaminyltransferase, MurG
IPR004276	Glycosyl transferase, family 28
IPR004182	GRAM
	SWISS-MODEL
	PDB Chain
	ModBase
	CATH Domain
	SCOP Domain

Publications Open in usermanual
1.	Campbell JA, Davies GJ, Bulone V, Henrissat B. A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities. Biochem. J. 326 ( Pt 3) 929-39 1997 [PubMed: 9334165] http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=9334165
2.	Henrissat B, Coutinho PM. Carbohydrate-Active Enzymes server. 1999

Additional Reading Open in usermanual
	Mulichak AM, Losey HC, Lu W, Wawrzak Z, Walsh CT, Garavito RM. Structure of the TDP-epi-vancosaminyltransferase GtfA from the chloroeremomycin biosynthetic pathway. Proc. Natl. Acad. Sci. U.S.A. 100 2003 9238-43 [PubMed: 12874381] http://dx.doi.org/10.1073/pnas.1233577100
	Ha S, Walker D, Shi Y, Walker S. The 1.9 A crystal structure of Escherichia coli MurG, a membrane-associated glycosyltransferase involved in peptidoglycan biosynthesis. Protein Sci. 9 2000 1045-52 [PubMed: 10892798] http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=10892798&action=stream&blobtype=pdf
	Hu Y, Chen L, Ha S, Gross B, Falcone B, Walker D, Mokhtarzadeh M, Walker S. Crystal structure of the MurG:UDP-GlcNAc complex reveals common structural principles of a superfamily of glycosyltransferases. Proc. Natl. Acad. Sci. U.S.A. 100 2003 845-9 [PubMed: 12538870] http://dx.doi.org/10.1073/pnas.0235749100
	Mulichak AM, Losey HC, Walsh CT, Garavito RM. Structure of the UDP-glucosyltransferase GtfB that modifies the heptapeptide aglycone in the biosynthesis of vancomycin group antibiotics. Structure 9 2001 547-57 [PubMed: 11470430] http://dx.doi.org/10.1016/S0969-2126(01)00616-5
	Mulichak AM, Lu W, Losey HC, Walsh CT, Garavito RM. Crystal structure of vancosaminyltransferase GtfD from the vancomycin biosynthetic pathway: interactions with acceptor and nucleotide ligands. Biochemistry 43 2004 5170-80 [PubMed: 15122882] http://dx.doi.org/10.1021/bi036130c
	Mengin-Lecreulx D, Texier L, Rousseau M, van Heijenoort J. The murG gene of Escherichia coli codes for the UDP-N-acetylglucosamine: N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase involved in the membrane steps of peptidoglycan synthesis. J. Bacteriol. 173 1991 4625-36 [PubMed: 1649817] http://ukpmc.ac.uk/picrender.cgi?tool=EBI&pubmedid=1649817&action=stream&blobtype=pdf

InterPro 23.1