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InterPro: IPR004206 mRNA capping enzyme, beta subunit

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129 proteins

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Accession

IPR004206 mRNA_capping_enz_bsu

Type

Family

Signatures Help

Database	ID	Name	Proteins
Gene3D	G3DSA:3.20.100.10	mRNA_capping_enz_bsu	103
Pfam	PF02940	mRNA_triPase	126
SuperFamily	SSF55154	mRNA_capping_enz_bsu	123
Signatures in BioMart

GO Term annotation Help

Process

GO:0006370 mRNA capping

Function

GO:0004651 polynucleotide 5'-phosphatase activity
GO:0005515 protein binding

Component

GO:0031533 mRNA cap methyltransferase complex

InterPro annotation

Entry Details in BioMart

Abstract

The mRNA capping enzyme in yeasts is composed of two separate subunits, a mRNA guanyltransferase and an RNA 5'-triphosphate. This is the beta subunit of mRNA capping enzyme which has triphosphatase activity [1, 2, 3]. The beta chain (polynucleotide 5'-phosphatase EC:3.1.3.33) converts the 5'-triphosphate end of a nascent mRNA chain into a diphosphate in the first step of mRNA capping. The function of the capping enzyme also depends on the guanylyltransferase activity conferred by the alpha chain (see IPR001339).

Structural links Help

PDB - click here

SCOP: d.63.1.1

CATH: 3.20.100.10

Database links Help

Enzyme: EC:3.1.3.33

PANDIT: PF02940

Blocks: IPB004206

Pfam Clan: CL0273.3

Taxonomic coverage Help

Example proteins Help

O13297 mRNA-capping enzyme subunit beta

O93803 mRNA-capping enzyme subunit beta

Q5UQX1 Probable mRNA-capping enzyme

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR004971	mRNA capping enzyme, large subunit
IPR004206	mRNA capping enzyme, beta subunit
IPR001339	mRNA capping enzyme
IPR016027	Nucleic acid-binding, OB-fold-like
	SWISS-MODEL
	PDB Chain
	ModBase
	SCOP Domain
	CATH Domain

Publications Open in usermanual
1.	Shibagaki Y, Gotoh H, Kato M, Mizumoto K. Localization and in vitro mutagenesis of the active site in the Saccharomyces cerevisiae mRNA capping enzyme. J. Biochem. 118 1303-9 1995 [PubMed: 8720151]
2.	Shibagaki Y, Itoh N, Yamada H, Nagata S, Mizumoto K. mRNA capping enzyme. Isolation and characterization of the gene encoding mRNA guanylytransferase subunit from Saccharomyces cerevisiae. J. Biol. Chem. 267 9521-8 1992 [PubMed: 1315757]
3.	Tsukamoto T, Shibagaki Y, Imajoh-Ohmi S, Murakoshi T, Suzuki M, Nakamura A, Gotoh H, Mizumoto K. Isolation and characterization of the yeast mRNA capping enzyme beta subunit gene encoding RNA 5'-triphosphatase, which is essential for cell viability. Biochem. Biophys. Res. Commun. 239 116-22 1997 [PubMed: 9345280] http://dx.doi.org/10.1006/bbrc.1997.7439

Additional Reading Open in usermanual
	Yamada-Okabe T, Mio T, Matsui M, Kashima Y, Arisawa M, Yamada-Okabe H. Isolation and characterization of the Candida albicans gene for mRNA 5'-triphosphatase: association of mRNA 5'-triphosphatase and mRNA 5'-guanylyltransferase activities is essential for the function of mRNA 5'-capping enzyme in vivo. FEBS Lett. 435 1998 49-54 [PubMed: 9755857] http://dx.doi.org/10.1016/S0014-5793(98)01037-0
	Lima CD, Wang LK, Shuman S. Structure and mechanism of yeast RNA triphosphatase: an essential component of the mRNA capping apparatus. Cell 99 1999 533-43 [PubMed: 10589681] http://dx.doi.org/10.1016/S0092-8674(00)81541-X

InterPro 23.1