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InterPro: IPR004103 Polysaccharide lyase family 8, C-terminal

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UniProtKB

Matches:

283 proteins

Overview:	sorted by AC,	sorted by name,	of known structure,	proteins with splice variants
Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
Table:	For all matching proteins,	of known structure
Architectures
Accession List
Matches in BioMart

Accession

IPR004103 Lyase_8_C

Type

Domain

Signatures Help

Database	ID	Name	Proteins
Gene3D	G3DSA:2.60.220.10	Lyase_8_C	201
Pfam	PF02884	Lyase_8_C	231
Signatures in BioMart

InterPro Relationships Help

Parent

IPR011071 Polysaccharide lyase family 8-like, C-terminal

GO Term annotation Help

Function

GO:0016829 lyase activity

Component

GO:0005576 extracellular region

InterPro annotation

Entry Details in BioMart

Abstract

Proteins containing this domain consist of a group of secreted bacterial lyase enzymes capable of acting on hyaluronan (hyaluronate lyase, EC:4.2.2.1) and chondroitin (chondroitin AC lyase, EC:4.2.2.5) in the extracellular matrix of host tissues, contributing to the invasive capacity of the pathogen [1, 2]. This domain is almost always associated with the polysaccharide lyase family 8, N-terminal domain (see IPR003159). This entry represents the C-terminal domain of hyaluronate and chondroitin AC lyase enzymes.

Structural links Help

PDB - click here

SCOP: b.24.1.1 , b.30.5.2

CATH: 2.60.220.10

Database links Help

Enzyme: EC:4.2.2

PANDIT: PF02884

Blocks: IPB004103

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR004103

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

P59807 Chondroitin ABC endolyase 1

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR014718	Glycoside hydrolase-type carbohydrate-binding, subgroup
IPR012329	Polysaccharide lyase family 8, N-terminal
IPR004103	Polysaccharide lyase family 8, C-terminal
IPR011013	Glycoside hydrolase-type carbohydrate-binding
IPR008929	Chondroitin AC/alginate lyase
IPR011071	Polysaccharide lyase family 8-like, C-terminal
IPR015177	Lyase, catalytic
IPR008979	Galactose-binding domain-like
IPR015176	Lyase, N-terminal
IPR003159	Polysaccharide lyase family 8, central domain
	PDB Chain
	ModBase
	CATH Domain
	SCOP Domain

Publications Open in usermanual
1.	Rigden DJ, Jedrzejas MJ. Structures of Streptococcus pneumoniae hyaluronate lyase in complex with chondroitin and chondroitin sulfate disaccharides. Insights into specificity and mechanism of action. J. Biol. Chem. 278 50596-606 2003 [PubMed: 14523022] http://dx.doi.org/10.1074/jbc.M307596200
2.	Fethiere J, Eggimann B, Cygler M. Crystal structure of chondroitin AC lyase, a representative of a family of glycosaminoglycan degrading enzymes. J. Mol. Biol. 288 635-47 1999 [PubMed: 10329169] http://dx.doi.org/10.1006/jmbi.1999.2698

Additional Reading Open in usermanual
	Maruyama Y, Mikami B, Hashimoto W, Murata K. A structural factor responsible for substrate recognition by Bacillus sp. GL1 xanthan lyase that acts specifically on pyruvated side chains of xanthan. Biochemistry 46 2007 781-91 [PubMed: 17223699] http://dx.doi.org/10.1021/bi0619775
	Rigden DJ, Littlejohn JE, Joshi HV, de Groot BL, Jedrzejas MJ. Alternate structural conformations of Streptococcus pneumoniae hyaluronan lyase: insights into enzyme flexibility and underlying molecular mechanism of action. J. Mol. Biol. 358 2006 1165-78 [PubMed: 16569416] http://dx.doi.org/10.1016/j.jmb.2006.02.066
	Farrell AM, Taylor D, Holland KT. Cloning, nucleotide sequence determination and expression of the Staphylococcus aureus hyaluronate lyase gene. FEMS Microbiol. Lett. 130 1995 81-5 [PubMed: 7557301] http://dx.doi.org/10.1016/0378-1097(95)00187-A
	Lunin VV, Li Y, Linhardt RJ, Miyazono H, Kyogashima M, Kaneko T, Bell AW, Cygler M. High-resolution crystal structure of Arthrobacter aurescens chondroitin AC lyase: an enzyme-substrate complex defines the catalytic mechanism. J. Mol. Biol. 337 2004 367-86 [PubMed: 15003453] http://dx.doi.org/10.1016/j.jmb.2003.12.071
	Rigden DJ, Botzki A, Nukui M, Mewbourne RB, Lamani E, Braun S, von Angerer E, Bernhardt G, Dove S, Buschauer A, Jedrzejas MJ. Design of new benzoxazole-2-thione-derived inhibitors of Streptococcus pneumoniae hyaluronan lyase: structure of a complex with a 2-phenylindole. Glycobiology 16 2006 757-65 [PubMed: 16638841] http://dx.doi.org/10.1093/glycob/cwj116
	Maruyama Y, Hashimoto W, Mikami B, Murata K. Crystal structure of Bacillus sp. GL1 xanthan lyase complexed with a substrate: insights into the enzyme reaction mechanism. J. Mol. Biol. 350 2005 974-86 [PubMed: 15979090] http://dx.doi.org/10.1016/j.jmb.2005.05.055

InterPro 23.1