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InterPro: IPR003385 Glycoside hydrolase, family 77

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1114 proteins

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Accession

IPR003385 Glyco_hydro_77

Type

Family

Signatures Help

Database	ID	Name	Proteins
Pfam	PF02446	Glyco_hydro_77	1114
TIGRFAMs	TIGR00217	malQ	962
Signatures in BioMart

InterPro Relationships Help

Contains

IPR013781 Glycoside hydrolase, subgroup, catalytic core

GO Term annotation Help

Process

GO:0005975 carbohydrate metabolic process

Function

GO:0004134 4-alpha-glucanotransferase activity

InterPro annotation

Entry Details in BioMart

Abstract

O-Glycosyl hydrolases EC:3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [1, 2, 3]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site [4]. Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in clans.

The enzymes in this entry (EC:2.4.1.25) belong to the glycoside hydrolase family 77 GH77, and transfer a segment of a (1,4)-alpha-D-glucan to a new 4-position in an acceptor, which may be glucose or (1,4)-alpha-D-glucan [5]. They belong to the disproportionating family of enzymes.

Structural links Help

PDB - click here

SCOP: c.1.8.1

CATH: 3.20.20.80

Database links Help

Enzyme: EC:2.4.1.25

CAZy: GH77

PANDIT: PF02446

Blocks: IPB003385

Pfam Clan: CL0058.12

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR003385

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

O66937 4-alpha-glucanotransferase

P72785 4-alpha-glucanotransferase

Q06801 4-alpha-glucanotransferase, chloroplastic/amyloplastic

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR013781	Glycoside hydrolase, subgroup, catalytic core
IPR017853	Glycoside hydrolase, catalytic core
IPR003385	Glycoside hydrolase, family 77
	SWISS-MODEL
	PDB Chain
	ModBase
	SCOP Domain
	CATH Domain

Publications Open in usermanual
1.	Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases. Proc. Natl. Acad. Sci. U.S.A. 92 7090-4 1995 [PubMed: 7624375] http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=7624375&action=stream&blobtype=pdf
2.	Davies G, Henrissat B. Structures and mechanisms of glycosyl hydrolases. Structure 3 853-9 1995 [PubMed: 8535779] http://dx.doi.org/10.1016/S0969-2126(01)00220-9
3.	Bairoch A. Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT. 1999
4.	Henrissat B, Coutinho PM. Carbohydrate-Active Enzymes server. 1999
5.	Takaha T, Yanase M, Okada S, Smith SM. Disproportionating enzyme (4-alpha-glucanotransferase; EC 2.4.1.25) of potato. Purification, molecular cloning, and potential role in starch metabolism. J. Biol. Chem. 268 1391-6 1993 [PubMed: 7678257] http://intl.jbc.org/cgi/reprint/268/2/1391.pdf

Additional Reading Open in usermanual
	Przylas I, Terada Y, Fujii K, Takaha T, Saenger W, Strater N. X-ray structure of acarbose bound to amylomaltase from Thermus aquaticus. Implications for the synthesis of large cyclic glucans. Eur. J. Biochem. 267 2000 6903-13 [PubMed: 11082203]
	Przylas I, Tomoo K, Terada Y, Takaha T, Fujii K, Saenger W, Strater N. Crystal structure of amylomaltase from thermus aquaticus, a glycosyltransferase catalysing the production of large cyclic glucans. J. Mol. Biol. 296 2000 873-86 [PubMed: 10677288] http://dx.doi.org/10.1006/jmbi.1999.3503
	Barends TR, Bultema JB, Kaper T, van der Maarel MJ, Dijkhuizen L, Dijkstra BW. Three-way stabilization of the covalent intermediate in amylomaltase, an alpha-amylase-like transglycosylase. J. Biol. Chem. 282 2007 17242-9 [PubMed: 17420245] http://dx.doi.org/10.1074/jbc.M701444200

InterPro 23.1