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InterPro: IPR002649 tRNA (guanine-N1-)-methyltransferase, bacteria

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UniProtKB

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1627 proteins

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Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
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Architectures
Accession List
Matches in BioMart

Accession

IPR002649 tRNA_m1G_MeTrfase_bac

Type

Family

Signatures Help

Database	ID	Name	Proteins
HAMAP	MF_00605	tRNA_m1G_MeTrfase_bac	1351
PIRSF	PIRSF000386	tRNA_mtase	1538
TIGRFAMs	TIGR00088	trmD	1599
Signatures in BioMart

InterPro Relationships Help

Parent

IPR016009 tRNA (guanine-N1-)-methyltransferase

GO Term annotation Help

Process

GO:0008033 tRNA processing

Function

GO:0003723 RNA binding
GO:0009019 tRNA (guanine-N1-)-methyltransferase activity

InterPro annotation

Entry Details in BioMart

Abstract

In transfer RNA many different modified nucleosides are found, especially in the anticodon region. tRNA (guanine-N1-)-methyltransferase EC:2.1.1.31 is one of several nucleases operating together with the tRNA-modifying enzymes before the formation of the mature tRNA. It catalyses the reaction:

S-adenosyl-L-methionine + tRNA -> S-adenosyl-L-homocysteine + tRNA containing N1-methylguanine

methylating guanosine(G) to N1-methylguanine (1-methylguanosine (m1G)) at position 37 of tRNAs that read CUN (leucine), CCN(proline), and CGG (arginine) codons. The presence of m1G improves the cellular growth rate and the polypeptide steptime and also prevents the tRNA from shifting the reading frame [1].

The mechanism of the trmD3-induced frameshift involving mutant tRNA(Pro) and tRNA(Leu) species has been investigated [2]. It has been suggested that the conformation of the anticodon loop may be a major determining element for the formation of m1G37 in vivo [3].

Structural links Help

PDB - click here

SCOP: c.116.1.4

CATH: 1.10.1270.20 , 3.40.1280.10

Database links Help

Enzyme: EC:2.1.1.31

Blocks: IPB002649

CluSTr: ALLvsALL:30714:62.6

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR002649

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

A5GMI5 tRNA (guanine-N(1)-)-methyltransferase

O67463 tRNA (guanine-N(1)-)-methyltransferase

P72828 tRNA (guanine-N(1)-)-methyltransferase

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR002649	tRNA (guanine-N1-)-methyltransferase, bacteria
IPR016009	tRNA (guanine-N1-)-methyltransferase
	SWISS-MODEL
	PDB Chain
	ModBase
	CATH Domain
	SCOP Domain

Publications Open in usermanual
1.	Hagervall TG, Ericson JU, Esberg KB, Li JN, Bjork GR. Role of tRNA modification in translational fidelity. Biochim. Biophys. Acta 1050 263-6 1990 [PubMed: 2207153] http://dx.doi.org/10.1016/0167-4781(90)90178-5
2.	Hagervall TG, Tuohy TM, Atkins JF, Bjork GR. Deficiency of 1-methylguanosine in tRNA from Salmonella typhimurium induces frameshifting by quadruplet translocation. J. Mol. Biol. 232 756-65 1993 [PubMed: 7689113] http://dx.doi.org/10.1006/jmbi.1993.1429
3.	Qian Q, Bjork GR. Structural requirements for the formation of 1-methylguanosine in vivo in tRNA(Pro)GGG of Salmonella typhimurium. J. Mol. Biol. 266 283-96 1997 [PubMed: 9047363] http://dx.doi.org/10.1006/jmbi.1996.0789

Additional Reading Open in usermanual
	Hjalmarsson KJ, Bystrom AS, Bjork GR. Purification and characterization of transfer RNA (guanine-1)methyltransferase from Escherichia coli. J. Biol. Chem. 258 1983 1343-51 [PubMed: 6337136] http://intl.jbc.org/cgi/reprint/258/2/1343.pdf
	Elkins PA, Watts JM, Zalacain M, van Thiel A, Vitazka PR, Redlak M, Andraos-Selim C, Rastinejad F, Holmes WM. Insights into catalysis by a knotted TrmD tRNA methyltransferase. J. Mol. Biol. 333 2003 931-49 [PubMed: 14583191] http://dx.doi.org/10.1016/j.jmb.2003.09.011
	O'Dwyer K, Watts JM, Biswas S, Ambrad J, Barber M, Brule H, Petit C, Holmes DJ, Zalacain M, Holmes WM. Characterization of Streptococcus pneumoniae TrmD, a tRNA methyltransferase essential for growth. J. Bacteriol. 186 2004 2346-54 [PubMed: 15060037] http://dx.doi.org/10.1128/JB.186.8.2346-2354.2004
	Liu J, Wang W, Shin DH, Yokota H, Kim R, Kim SH. Crystal structure of tRNA (m1G37) methyltransferase from Aquifex aeolicus at 2.6 A resolution: a novel methyltransferase fold. Proteins 53 2003 326-8 [PubMed: 14517984] http://dx.doi.org/10.1002/prot.10479
	Ahn HJ, Kim HW, Yoon HJ, Lee BI, Suh SW, Yang JK. Crystal structure of tRNA(m1G37)methyltransferase: insights into tRNA recognition. EMBO J. 22 2003 2593-603 [PubMed: 12773376] http://dx.doi.org/10.1093/emboj/cdg269

InterPro 23.1