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InterPro: IPR002575 Aminoglycoside phosphotransferase

Protein matches Help

UniProtKB

Matches:

7264 proteins

Overview:	sorted by AC,	sorted by name,	of known structure,	proteins with splice variants
Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
Table:	For all matching proteins,	of known structure
Architectures
Accession List
Matches in BioMart

Accession

IPR002575 APH_trans

Type

Domain

Signatures Help

Database	ID	Name	Proteins
Pfam	PF01636	APH	7264
Signatures in BioMart

InterPro Relationships Help

Parent

IPR011009 Protein kinase-like domain

Children

IPR005280 Homoserine kinase ThrB
IPR009212 Methylthioribose kinase
IPR014093 Thiamine kinase
IPR014255 Spore coat protein CotS
IPR016259 Hygromycin-B kinase

Found in

IPR003547 Serine/threonine protein kinase, yersinia
IPR012180 Bifunctional ATPase/phosphotransferase, cell wall biosynthesis
IPR012811 Trehalose synthase/probable maltokinase, C-terminal

Contains

IPR020659 Serine/threonine-protein kinase, predicted

InterPro annotation

Entry Details in BioMart

Abstract

This entry consists of bacterial antibiotic resistance proteins, which confer resistance to various aminoglycosides they include:- aminoglycoside 3'-phosphotransferase or kanamycin kinase / neomycin-kanamycin phosphotransferase and streptomycin 3''-kinase or streptomycin 3''-phosphotransferase. The aminoglycoside phosphotransferases inactivate aminoglycoside antibiotics via phosphorylation [1].

Structural links Help

PDB - click here

SCOP: d.144.1.6

CATH: 3.30.200.20 , 3.90.1200.10

Database links Help

Enzyme: EC:2.7.1

PANDIT: PF01636

Blocks: IPB002575

Pfam Clan: CL0016.18

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR002575

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

A5PJU6 Aminoglycoside phosphotransferase domain-containing protein 1

O31663 Methylthioribose kinase

P73341 Uncharacterized protein sll1119

Q5U5V2 Aminoglycoside phosphotransferase domain-containing protein 1

Q709F0 Acyl-CoA dehydrogenase family member 11

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR009075	Acyl-CoA dehydrogenase/oxidase C-terminal
IPR009100	Acyl-CoA dehydrogenase/oxidase
IPR013786	Acyl-CoA dehydrogenase/oxidase, N-terminal
IPR006092	Acyl-CoA dehydrogenase, N-terminal
IPR006090	Acyl-CoA oxidase/dehydrogenase, type 1
IPR006091	Acyl-CoA oxidase/dehydrogenase, central domain
IPR013764	Acyl-CoA oxidase/dehydrogenase, type1/2, C-terminal
IPR011009	Protein kinase-like domain
IPR002575	Aminoglycoside phosphotransferase
IPR009212	Methylthioribose kinase
	PDB Chain
	ModBase
	SWISS-MODEL
	SCOP Domain

Publications Open in usermanual
1.	Trower MK, Clark KG. PCR cloning of a streptomycin phosphotransferase (aphE) gene from Streptomyces griseus ATCC 12475. Nucleic Acids Res. 18 4615 1990 [PubMed: 2167474] http://nar.oxfordjournals.org/cgi/content/abstract/18/15/4615

Additional Reading Open in usermanual
	Ku SY, Smith GD, Howell PL. ADP-2Ho as a phasing tool for nucleotide-containing proteins. Acta Crystallogr. D Biol. Crystallogr. 63 2007 493-9 [PubMed: 17372354] http://dx.doi.org/10.1107/S0907444907006592
	Kohl A, Amstutz P, Parizek P, Binz HK, Briand C, Capitani G, Forrer P, Pluckthun A, Grutter MG. Allosteric inhibition of aminoglycoside phosphotransferase by a designed ankyrin repeat protein. Structure 13 2005 1131-41 [PubMed: 16084385] http://dx.doi.org/10.1016/j.str.2005.04.020
	Sarwar M, Akhtar M. Cloning of aminoglycoside phosphotransferase (APH) gene from antibiotic-producing strain of Bacillus circulans into a high-expression vector, pKK223-3. Purification, properties and location of the enzyme. Biochem. J. 268 1990 671-7 [PubMed: 2163618] http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=2163618&action=stream&blobtype=pdf
	Zheng J, He C, Singh VK, Martin NL, Jia Z. Crystal structure of a novel prokaryotic Ser/Thr kinase and its implication in the Cpx stress response pathway. Mol. Microbiol. 63 2007 1360-71 [PubMed: 17302814] http://dx.doi.org/10.1111/j.1365-2958.2007.05611.x
	Ku SY, Yip P, Cornell KA, Riscoe MK, Behr JB, Guillerm G, Howell PL. Structures of 5-methylthioribose kinase reveal substrate specificity and unusual mode of nucleotide binding. J. Biol. Chem. 282 2007 22195-206 [PubMed: 17522047] http://dx.doi.org/10.1074/jbc.M611045200
	Nurizzo D, Shewry SC, Perlin MH, Brown SA, Dholakia JN, Fuchs RL, Deva T, Baker EN, Smith CA. The crystal structure of aminoglycoside-3'-phosphotransferase-IIa, an enzyme responsible for antibiotic resistance. J. Mol. Biol. 327 2003 491-506 [PubMed: 12628253] http://dx.doi.org/10.1016/S0022-2836(03)00121-9

InterPro 23.1