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InterPro: IPR002173 Carbohydrate/puine kinase, PfkB, conserved site

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6412 proteins

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Accession

IPR002173 Carboh/pur_kinase_PfkB_CS

Type

Conserved_site

Signatures Help

Database	ID	Name	Proteins
PROSITE pattern	PS00583	PFKB_KINASES_1	2763
PROSITE pattern	PS00584	PFKB_KINASES_2	5349
Signatures in BioMart

InterPro Relationships Help

Found in

IPR001805 Adenosine kinase
IPR005926 Tagatose-6-phosphate kinase
IPR011611 Carbohydrate/purine kinase
IPR011877 Ribokinase, bacterial
IPR011913 RfaE bifunctional protein, domain I
IPR017583 1-phosphofructokinase

InterPro annotation

Entry Details in BioMart

Abstract

It has been shown [1, 2, 3] that the following carbohydrate and purine kinases are evolutionary related and can be grouped into a single family, which is known [1] as the 'pfkB family':

Fructokinase (EC:2.7.1.4) (gene scrK).
6-phosphofructokinase isozyme 2 (EC:2.7.1.11) (phosphofructokinase-2) (gene pfkB). pfkB is a minor phosphofructokinase isozyme in Escherichia coli and is not evolutionary related to the major isozyme (gene pfkA). Plant 6-phosphofructokinase also belong to this family.
Ribokinase (EC:2.7.1.15) (gene rbsK).
Adenosine kinase (EC:2.7.1.20) (gene ADK).
2-dehydro-3-deoxygluconokinase (EC:2.7.1.45) (gene: kdgK).
1-phosphofructokinase (EC:2.7.1.56) (fructose 1-phosphate kinase) (gene fruK).
Inosine-guanosine kinase (EC:2.7.1.73) (gene gsk).
Tagatose-6-phosphate kinase (EC:2.7.1.144) (phosphotagatokinase) (gene lacC).
E. coli hypothetical protein yeiC.
E. coli hypothetical protein yeiI.
E. coli hypothetical protein yhfQ.
E. coli hypothetical protein yihV.
Yeast hypothetical protein YJR105w.

All the above kinases are proteins of from 280 to 430 amino acid residues that share a few region of sequence similarity.

Note: some bacterial fructokinases belong to the ROK family (see IPR000600).

Structural links Help

PDB - click here

SCOP: c.72.1.1

CATH: 3.40.1190.20

Database links Help

PDBe-motif: PS00583 , PS00584

Enzyme: EC:2.7.1

PROSITE doc: PDOC00504

Blocks: IPB002173

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR002173

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

A2WXV8 Fructokinase-1

O82616 Putative fructokinase-5

P25332 Probable ribokinase

P55263 Adenosine kinase

P55264 Adenosine kinase

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR002173	Carbohydrate/puine kinase, PfkB, conserved site
IPR011611	Carbohydrate/purine kinase
IPR002139	Ribokinase
IPR001805	Adenosine kinase
	SWISS-MODEL
	PDB Chain
	ModBase
	SCOP Domain
	CATH Domain

Publications Open in usermanual
1.	Wu LF, Reizer A, Reizer J, Cai B, Tomich JM, Saier MH Jr. Nucleotide sequence of the Rhodobacter capsulatus fruK gene, which encodes fructose-1-phosphate kinase: evidence for a kinase superfamily including both phosphofructokinases of Escherichia coli. J. Bacteriol. 173 3117-27 1991 [PubMed: 1850730] http://jb.asm.org/cgi/content/abstract/173/10/3117
2.	Orchard LM, Kornberg HL. Sequence similarities between the gene specifying 1-phosphofructokinase (fruK), genes specifying other kinases in Escherichia coli K12, and lacC of Staphylococcus aureus. Proc. Biol. Sci. 242 87-90 1990 [PubMed: 1981619] http://www.journals.royalsoc.ac.uk/openurl.asp?genre=article&issn=0962-8452&volume=242&issue=1304&spage=87
3.	Blatch GL, Scholle RR, Woods DR. Nucleotide sequence and analysis of the Vibrio alginolyticus sucrose uptake-encoding region. Gene 95 17-23 1990 [PubMed: 2174811] http://dx.doi.org/10.1016/0378-1119(90)90408-J

Additional Reading Open in usermanual
	Zhang Y, El Kouni MH, Ealick SE. Structure of Toxoplasma gondii adenosine kinase in complex with an ATP analog at 1.1 angstroms resolution. Acta Crystallogr. D Biol. Crystallogr. 62 2006 140-5 [PubMed: 16421444] http://dx.doi.org/10.1107/S090744490503430X
	Inagaki E, Ukita Y, Kumei M, Kajihara Y, Tahirov TH. Crystallization and preliminary crystallographic analysis of 2-keto-3-deoxygluconate kinase from Thermus thermophilus. Acta Crystallogr. D Biol. Crystallogr. 60 2004 761-3 [PubMed: 15039578] http://dx.doi.org/10.1107/S0907444904002665
	Muchmore SW, Smith RA, Stewart AO, Cowart MD, Gomtsyan A, Matulenko MA, Yu H, Severin JM, Bhagwat SS, Lee CH, Kowaluk EA, Jarvis MF, Jakob CL. Crystal structures of human adenosine kinase inhibitor complexes reveal two distinct binding modes. J. Med. Chem. 49 2006 6726-31 [PubMed: 17154503] http://dx.doi.org/10.1021/jm060189a
	Zhang Y, El Kouni MH, Ealick SE. Substrate analogs induce an intermediate conformational change in Toxoplasma gondii adenosine kinase. Acta Crystallogr. D Biol. Crystallogr. 63 2007 126-34 [PubMed: 17242506] http://dx.doi.org/10.1107/S0907444906043654
	Ohshima N, Inagaki E, Yasuike K, Takio K, Tahirov TH. Structure of Thermus thermophilus 2-Keto-3-deoxygluconate kinase: evidence for recognition of an open chain substrate. J. Mol. Biol. 340 2004 477-89 [PubMed: 15210349] http://dx.doi.org/10.1016/j.jmb.2004.04.074

InterPro 23.1