EBI Databases InterPro	Search Open in usermanual InterPro:
Jump to: InterProScan Databases Documentation FTP site Help Click on the icon for context sensitive help from the user manual. Advanced search

InterPro: IPR001805 Adenosine kinase

Protein matches Help

UniProtKB

Matches:

322 proteins

Overview:	sorted by AC,	sorted by name,	of known structure,	proteins with splice variants
Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
Table:	For all matching proteins,	of known structure
Architectures
Accession List
Matches in BioMart

Accession

IPR001805 Adenokinase

Type

Family

Signatures Help

Database	ID	Name	Proteins
PRINTS	PR00989	ADENOKINASE	218
PANTHER	PTHR10584:SF24	Adenokinase	316
Signatures in BioMart

InterPro Relationships Help

Contains

IPR002173 Carbohydrate/puine kinase, PfkB, conserved site
IPR011611 Carbohydrate/purine kinase

GO Term annotation Help

Process

GO:0006166 purine ribonucleoside salvage

Function

GO:0004001 adenosine kinase activity

InterPro annotation

Entry Details in BioMart

Abstract

Adenosine kinase (ADK) phosphorylates adenosine and other related nucleosides [1, 2]. It is exclusive to eukaryotes [3] and is a key enzyme in the purine salvage pathway. ADK catalyses the reaction:

Adenosine + ATP = ADP + AMP

This reaction prevents toxic levels of adenosine building up within the cell. Experiments have been carried out on Gallus gallus (chicken) embryonic neurons to document the toxicity of adenosine, and the associated requirement for ADK [4].

Adenosine and ATP have been connected with pain, and the control of pain [5, 6]. Associated with this, adenosine has also been shown to have a number of anti-inflammatory activities [7]. Human ADK bears little sequence similarity to other mammalian nucleoside kinases, but does share high similarity with some microbrial ribokinases and fructokinases, suggesting possible evolutionary connections [1, 2].

Structural links Help

PDB - click here

SCOP: c.72.1.1

CATH: 3.30.1110.10 , 3.40.1190.20

Database links Help

Enzyme: EC:2.7.1.20

Blocks: IPB001805

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR001805

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

P47143 Adenosine kinase

P55262 Adenosine kinase

P55263 Adenosine kinase

P55264 Adenosine kinase

Q9LZG0 Adenosine kinase 2

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR002173	Carbohydrate/puine kinase, PfkB, conserved site
IPR011611	Carbohydrate/purine kinase
IPR001805	Adenosine kinase
	SWISS-MODEL
	PDB Chain
	ModBase
	SCOP Domain
	CATH Domain

Publications Open in usermanual
1.	Spychala J, Datta NS, Takabayashi K, Datta M, Fox IH, Gribbin T, Mitchell BS. Cloning of human adenosine kinase cDNA: sequence similarity to microbial ribokinases and fructokinases. Proc. Natl. Acad. Sci. U.S.A. 93 1232-7 1996 [PubMed: 8577746] http://dx.doi.org/10.1073/pnas.93.3.1232
2.	Singh B, Hao W, Wu Z, Eigl B, Gupta RS. Cloning and characterization of cDNA for adenosine kinase from mammalian (Chinese hamster, mouse, human and rat) species. High frequency mutants of Chinese hamster ovary cells involve structural alterations in the gene. Eur. J. Biochem. 241 564-71 1996 [PubMed: 8917457] http://dx.doi.org/10.1111/j.1432-1033.1996.00564.x
3.	McNally T, Helfrich RJ, Cowart M, Dorwin SA, Meuth JL, Idler KB, Klute KA, Simmer RL, Kowaluk EA, Halbert DN. Cloning and expression of the adenosine kinase gene from rat and human tissues. Biochem. Biophys. Res. Commun. 231 645-50 1997 [PubMed: 9070863] http://dx.doi.org/10.1006/bbrc.1997.6157
4.	Kulkarni JS, Przywara DA, Wakade TD, Wakade AR. Adenosine induces apoptosis by inhibiting mRNA and protein synthesis in chick embryonic sympathetic neurons. Neurosci. Lett. 248 187-90 1998 [PubMed: 9654340] http://dx.doi.org/10.1016/S0304-3940(98)00369-3
5.	Keil GJ, Salter MW. Multiple roles of ATP and adenosine in somatosensory processing: Therapeutic implications. Drug Dev. Res. 39 279-88 1996
6.	Sawynok J. Adenosine receptor activation and nociception. Eur. J. Pharmacol. 347 1-11 1998 [PubMed: 9650842] http://dx.doi.org/10.1016/S0014-2999(97)01605-1
7.	Firestein GS. Anti-inflammatory effects of adenosine kinase inhibitors in acute and chronic inflammation. Drug Dev. Res. 39 371-6 1996

Additional Reading Open in usermanual
	Zhang Y, El Kouni MH, Ealick SE. Structure of Toxoplasma gondii adenosine kinase in complex with an ATP analog at 1.1 angstroms resolution. Acta Crystallogr. D Biol. Crystallogr. 62 2006 140-5 [PubMed: 16421444] http://dx.doi.org/10.1107/S090744490503430X
	Schumacher MA, Scott DM, Mathews II, Ealick SE, Roos DS, Ullman B, Brennan RG. Crystal structures of Toxoplasma gondii adenosine kinase reveal a novel catalytic mechanism and prodrug binding. J. Mol. Biol. 298 2000 875-93 [PubMed: 10801355] http://dx.doi.org/10.1006/jmbi.2000.3753
	Muchmore SW, Smith RA, Stewart AO, Cowart MD, Gomtsyan A, Matulenko MA, Yu H, Severin JM, Bhagwat SS, Lee CH, Kowaluk EA, Jarvis MF, Jakob CL. Crystal structures of human adenosine kinase inhibitor complexes reveal two distinct binding modes. J. Med. Chem. 49 2006 6726-31 [PubMed: 17154503] http://dx.doi.org/10.1021/jm060189a
	Zhang Y, El Kouni MH, Ealick SE. Substrate analogs induce an intermediate conformational change in Toxoplasma gondii adenosine kinase. Acta Crystallogr. D Biol. Crystallogr. 63 2007 126-34 [PubMed: 17242506] http://dx.doi.org/10.1107/S0907444906043654
	Cook WJ, DeLucas LJ, Chattopadhyay D. Crystal structure of adenosine kinase from Toxoplasma gondii at 1.8 A resolution. Protein Sci. 9 2000 704-12 [PubMed: 10794412] http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=10794412&action=stream&blobtype=pdf

InterPro 23.1