EBI Databases InterPro	Search Open in usermanual InterPro:
Jump to: InterProScan Databases Documentation FTP site Help Click on the icon for context sensitive help from the user manual. Advanced search

InterPro: IPR001339 mRNA capping enzyme

Protein matches Help

UniProtKB

Matches:

215 proteins

Overview:	sorted by AC,	sorted by name,	of known structure,	proteins with splice variants
Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
Table:	For all matching proteins,	of known structure
Architectures
Accession List
Matches in BioMart

Accession

IPR001339 mRNA_cap_enzyme

Type

Domain

Signatures Help

Database	ID	Name	Proteins
Pfam	PF01331	mRNA_cap_enzyme	215
Signatures in BioMart

InterPro Relationships Help

Found in

IPR017074 mRNA capping enzyme, bifunctional
IPR017075 mRNA capping enzyme, alpha subunit

GO Term annotation Help

Process

GO:0006370 mRNA capping
GO:0006397 mRNA processing

Function

GO:0004484 mRNA guanylyltransferase activity

InterPro annotation

Entry Details in BioMart

Abstract

The mRNA capping enzyme in yeasts is composed of two separate chains, alpha a mRNA guanyltransferase and beta an RNA 5'-triphosphate. X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes [1]. Binding of the enzyme to nucleotides is specific to the GMP moiety of GTP. The viral mRNA capping enzyme is a monomer that transfers a GMP cap onto the end of mRNA that terminates with a 5'-diphosphate tail.

Structural links Help

PDB - click here

SCOP: d.142.2.3

CATH: 3.90.63.10 , 4.10.87.10

Database links Help

Enzyme: EC:2.7.7.50

PANDIT: PF01331

Blocks: IPB001339

Pfam Clan: CL0078.9

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR001339

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

O55236 mRNA-capping enzyme

O60942 mRNA-capping enzyme

Q01159 mRNA-capping enzyme subunit alpha

Q17607 mRNA-capping enzyme

Q5UQX1 Probable mRNA-capping enzyme

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR013846	mRNA capping enzyme, C-terminal
IPR004971	mRNA capping enzyme, large subunit
IPR017075	mRNA capping enzyme, alpha subunit
IPR004206	mRNA capping enzyme, beta subunit
IPR017074	mRNA capping enzyme, bifunctional
IPR000387	Dual-specific/protein-tyrosine phosphatase, conserved region
IPR016027	Nucleic acid-binding, OB-fold-like
IPR016130	Protein-tyrosine phosphatase, active site
IPR000340	Dual specificity phosphatase, catalytic domain
IPR012340	Nucleic acid-binding, OB-fold
IPR001339	mRNA capping enzyme
	PDB Chain
	ModBase
	CATH Domain
	SWISS-MODEL
	SCOP Domain

Publications Open in usermanual
1.	Hakansson K, Doherty AJ, Shuman S, Wigley DB. X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes. Cell 89 545-53 1997 [PubMed: 9160746] http://dx.doi.org/10.1016/S0092-8674(00)80236-6

Additional Reading Open in usermanual
	Fabrega C, Shen V, Shuman S, Lima CD. Structure of an mRNA capping enzyme bound to the phosphorylated carboxy-terminal domain of RNA polymerase II. Mol. Cell 11 2003 1549-61 [PubMed: 12820968] http://dx.doi.org/10.1016/S1097-2765(03)00187-4
	Hakansson K, Wigley DB. Structure of a complex between a cap analogue and mRNA guanylyl transferase demonstrates the structural chemistry of RNA capping. Proc. Natl. Acad. Sci. U.S.A. 95 1998 1505-10 [PubMed: 9465045] http://dx.doi.org/10.1073/pnas.95.4.1505

InterPro 23.1