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InterPro: IPR000805 Glycoside hydrolase, family 26

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UniProtKB

Matches:

227 proteins

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Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
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Accession

IPR000805 Glyco_hydro_26

Type

Family

Signatures Help

Database	ID	Name	Proteins
Pfam	PF02156	Glyco_hydro_26	224
PRINTS	PR00739	GLHYDRLASE26	181
Signatures in BioMart

InterPro Relationships Help

Children

IPR016714 Mannan endo-1,4-beta-mannosidase

GO Term annotation Help

Process

GO:0006080 substituted mannan metabolic process

Function

GO:0016985 mannan endo-1,4-beta-mannosidase activity

InterPro annotation

Entry Details in BioMart

Abstract

O-Glycosyl hydrolases EC:3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [1, 2, 3]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site [4]. Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in clans.

Glycoside hydrolase family 26 GH26 comprises enzymes with only one known activity; mannanase (EC:3.2.1.78).

Family 26 encompasses mainly mannan endo-1,4-beta-mannosidases. Mannan endo-1,4-beta-mannosidase hydrolyses mannan and galactomannan, but displays little activity towards other plant cell wall polysaccharides [5]. The enzyme randomly hydrolyses 1,4-beta-D-linkages in mannans, galacto-mannans, glucomannans and galactoglucomannans.

Structural links Help

PDB - click here

SCOP: c.1.8.3

CATH: 3.20.20.80

Database links Help

Enzyme: EC:3.2.1.78

CAZy: GH26

PANDIT: PF02156

Blocks: IPB000805

Pfam Clan: CL0058.12

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR000805

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

O05512 Mannan endo-1,4-beta-mannosidase

P55296 Mannan endo-1,4-beta-mannosidase A

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR000805	Glycoside hydrolase, family 26
IPR013781	Glycoside hydrolase, subgroup, catalytic core
IPR008979	Galactose-binding domain-like
IPR016714	Mannan endo-1,4-beta-mannosidase
IPR017853	Glycoside hydrolase, catalytic core
IPR009034	Dockering, cellulose docking
IPR002883	Dockerin cellulose-binding domain
IPR005084	Carbohydrate binding domain, family 6
	SWISS-MODEL
	PDB Chain
	ModBase

Publications Open in usermanual
1.	Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases. Proc. Natl. Acad. Sci. U.S.A. 92 7090-4 1995 [PubMed: 7624375] http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=7624375&action=stream&blobtype=pdf
2.	Davies G, Henrissat B. Structures and mechanisms of glycosyl hydrolases. Structure 3 853-9 1995 [PubMed: 8535779] http://dx.doi.org/10.1016/S0969-2126(01)00220-9
3.	Bairoch A. Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT. 1999
4.	Henrissat B, Coutinho PM. Carbohydrate-Active Enzymes server. 1999
5.	Braithwaite KL, Black GW, Hazlewood GP, Ali BR, Gilbert HJ. A non-modular endo-beta-1,4-mannanase from Pseudomonas fluorescens subspecies cellulosa. Biochem. J. 305 ( Pt 3) 1005-10 1995 [PubMed: 7848261] http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=7848261&action=stream&blobtype=pdf

Additional Reading Open in usermanual
	Ducros VM, Zechel DL, Murshudov GN, Gilbert HJ, Szabo L, Stoll D, Withers SG, Davies GJ. Substrate distortion by a beta-mannanase: snapshots of the Michaelis and covalent-intermediate complexes suggest a B(2,5) conformation for the transition state. Angew. Chem. Int. Ed. Engl. 41 2002 2824-7 [PubMed: 12203498] http://dx.doi.org/10.1002/1521-3773(20020802)41:15<2824::AID-ANIE2824>3.0.CO;2-G
	Money VA, Smith NL, Scaffidi A, Stick RV, Gilbert HJ, Davies GJ. Substrate distortion by a lichenase highlights the different conformational itineraries harnessed by related glycoside hydrolases. Angew. Chem. Int. Ed. Engl. 45 2006 5136-40 [PubMed: 16823793] http://dx.doi.org/10.1002/anie.200600802
	Jahn M, Stoll D, Warren RA, Szabo L, Singh P, Gilbert HJ, Ducros VM, Davies GJ, Withers SG. Expansion of the glycosynthase repertoire to produce defined manno-oligosaccharides. Chem. Commun. (Camb.) 2003 1327-9 [PubMed: 12841226] http://dx.doi.org/10.1039/b302380j
	Taylor EJ, Goyal A, Guerreiro CI, Prates JA, Money VA, Ferry N, Morland C, Planas A, Macdonald JA, Stick RV, Gilbert HJ, Fontes CM, Davies GJ. How family 26 glycoside hydrolases orchestrate catalysis on different polysaccharides: structure and activity of a Clostridium thermocellum lichenase, CtLic26A. J. Biol. Chem. 280 2005 32761-7 [PubMed: 15987675] http://dx.doi.org/10.1074/jbc.M506580200
	Le Nours J, Anderson L, Stoll D, Stalbrand H, Lo Leggio L. The structure and characterization of a modular endo-beta-1,4-mannanase from Cellulomonas fimi. Biochemistry 44 2005 12700-8 [PubMed: 16171384] http://dx.doi.org/10.1021/bi050779v

InterPro 23.1