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InterPro: IPR000757 Glycoside hydrolase, family 16

Protein matches Help

UniProtKB

Matches:

2022 proteins

Overview:	sorted by AC,	sorted by name,	of known structure,	proteins with splice variants
Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
Table:	For all matching proteins,	of known structure
Architectures
Accession List
Matches in BioMart

Accession

IPR000757 Glyco_hydro_16

Type

Domain

Signatures Help

Database	ID	Name	Proteins
Pfam	PF00722	Glyco_hydro_16	2022
Signatures in BioMart

InterPro Relationships Help

Parent

IPR013320 Concanavalin A-like lectin/glucanase, subgroup

Found in

IPR008264 Beta-glucanase
IPR016455 Xyloglucan endotransglucosylase/hydrolase
IPR017168 Glycoside hydrolase, family 16, CRH1, predicted

Contains

IPR008263 Glycoside hydrolase, family 16, active site

GO Term annotation Help

Process

GO:0005975 carbohydrate metabolic process

Function

GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

InterPro annotation

Entry Details in BioMart

Abstract

O-Glycosyl hydrolases EC:3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [1, 2, 3]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site [4]. Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in clans.

Glycoside hydrolase family 16 GH16 comprises enzymes with a number of known activities; lichenase (EC:3.2.1.73); xyloglucan xyloglucosyltransferase (EC:2.4.1.207); agarase (EC:3.2.1.81); kappa-carrageenase (EC:3.2.1.83); endo-beta-1,3-glucanase (EC:3.2.1.39); endo-beta-1,3-1,4-glucanase (EC:3.2.1.6); endo-beta-galactosidase (EC:3.2.1.103).

Structural links Help

PDB - click here

SCOP: b.29.1.2

CATH: 2.60.120.200

Database links Help

CAZy: GH16

PANDIT: PF00722

Pfam Clan: CL0004.16

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR000757

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

O80803 Probable xyloglucan endotransglucosylase/hydrolase protein 17

P17989 Beta-glucanase

P32623 Probable glycosidase CRH2

Q76BW5 Xyloglucan endotransglycosylase/hydrolase protein 8

Q9NHA8 Gram-negative bacteria-binding protein 3

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR017168	Glycoside hydrolase, family 16, CRH1, predicted
IPR016455	Xyloglucan endotransglucosylase/hydrolase
IPR013320	Concanavalin A-like lectin/glucanase, subgroup
IPR010713	Xyloglucan endo-transglycosylase, C-terminal
IPR008264	Beta-glucanase
IPR008263	Glycoside hydrolase, family 16, active site
IPR008985	Concanavalin A-like lectin/glucanase
IPR000757	Glycoside hydrolase, family 16
	PDB Chain
	ModBase
	CATH Domain
	SWISS-MODEL
	SCOP Domain

Publications Open in usermanual
1.	Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases. Proc. Natl. Acad. Sci. U.S.A. 92 7090-4 1995 [PubMed: 7624375] http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=7624375&action=stream&blobtype=pdf
2.	Davies G, Henrissat B. Structures and mechanisms of glycosyl hydrolases. Structure 3 853-9 1995 [PubMed: 8535779] http://dx.doi.org/10.1016/S0969-2126(01)00220-9
3.	Bairoch A. Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT. 1999
4.	Henrissat B, Coutinho PM. Carbohydrate-Active Enzymes server. 1999

Additional Reading Open in usermanual
	Gaiser OJ, Piotukh K, Ponnuswamy MN, Planas A, Borriss R, Heinemann U. Structural basis for the substrate specificity of a Bacillus 1,3-1,4-beta-glucanase. J. Mol. Biol. 357 2006 1211-25 [PubMed: 16483609] http://dx.doi.org/10.1016/j.jmb.2006.01.014
	Tempel W, Liu ZJ, Horanyi PS, Deng L, Lee D, Newton MG, Rose JP, Ashida H, Li SC, Li YT, Wang BC. Three-dimensional structure of GlcNAcalpha1-4Gal releasing endo-beta-galactosidase from Clostridium perfringens. Proteins 59 2005 141-4 [PubMed: 15688452] http://dx.doi.org/10.1002/prot.20363
	Allouch J, Helbert W, Henrissat B, Czjzek M. Parallel substrate binding sites in a beta-agarase suggest a novel mode of action on double-helical agarose. Structure 12 2004 623-32 [PubMed: 15062085] http://dx.doi.org/10.1016/j.str.2004.02.020
	Johansson P, Brumer H 3rd, Baumann MJ, Kallas AM, Henriksson H, Denman SE, Teeri TT, Jones TA. Crystal structures of a poplar xyloglucan endotransglycosylase reveal details of transglycosylation acceptor binding. Plant Cell 16 2004 874-86 [PubMed: 15020748] http://dx.doi.org/10.1105/tpc.020065
	Tsai LC, Shyur LF, Cheng YS, Lee SH. Crystal structure of truncated Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase in complex with beta-1,3-1,4-cellotriose. J. Mol. Biol. 354 2005 642-51 [PubMed: 16246371] http://dx.doi.org/10.1016/j.jmb.2005.09.041

InterPro 23.1