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InterPro: IPR000514 Glycoside hydrolase, family 39

Protein matches Help

UniProtKB

Matches:

160 proteins

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Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
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Accession List
Matches in BioMart

Accession

IPR000514 Glyco_hydro_39

Type

Family

Signatures Help

Database	ID	Name	Proteins
Pfam	PF01229	Glyco_hydro_39	160
PRINTS	PR00745	GLHYDRLASE39	114
PROSITE pattern	PS01027	GLYCOSYL_HYDROL_F39	67
Signatures in BioMart

InterPro Relationships Help

Contains

IPR013781 Glycoside hydrolase, subgroup, catalytic core

GO Term annotation Help

Process

GO:0005975 carbohydrate metabolic process

Function

GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

InterPro annotation

Entry Details in BioMart

Abstract

O-Glycosyl hydrolases EC:3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [1, 2, 3]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site [4]. Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in clans.

Glycoside hydrolase family 39 GH39 comprises enzymes with several known activities; alpha-L-iduronidase (EC:3.2.1.76); beta-xylosidase (EC:3.2.1.37).

The most highly conserved regions in these enzymes are located in their N-terminal sections. These contain a glutamic acid residue which, on the basis of similarities with other families of glycosyl hydrolases [1], probably acts as the proton donor in their catalytic mechanism.

Structural links Help

PDB - click here

SCOP: b.71.1.2 , c.1.8.3

CATH: 2.60.40.1500 , 3.20.20.80

Database links Help

PDBe-motif: PS01027

Enzyme: EC:3.2.1

CAZy: GH39

PROSITE doc: PDOC00787

PANDIT: PF01229

Blocks: IPB000514

Pfam Clan: CL0058.12

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR000514

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

P35475 Alpha-L-iduronidase

P36906 Beta-xylosidase

P48441 Alpha-L-iduronidase

Q01634 Alpha-L-iduronidase

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR008957	Fibronectin, type III-like fold
IPR013781	Glycoside hydrolase, subgroup, catalytic core
IPR017853	Glycoside hydrolase, catalytic core
IPR000514	Glycoside hydrolase, family 39
	PDB Chain
	ModBase
	SCOP Domain
	CATH Domain

Publications Open in usermanual
1.	Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases. Proc. Natl. Acad. Sci. U.S.A. 92 7090-4 1995 [PubMed: 7624375] http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=7624375&action=stream&blobtype=pdf
2.	Davies G, Henrissat B. Structures and mechanisms of glycosyl hydrolases. Structure 3 853-9 1995 [PubMed: 8535779] http://dx.doi.org/10.1016/S0969-2126(01)00220-9
3.	Bairoch A. Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT. 1999
4.	Henrissat B, Coutinho PM. Carbohydrate-Active Enzymes server. 1999

Additional Reading Open in usermanual
	Czjzek M, Bravman T, Henrissat B, Shoham Y. Crystallization and preliminary X-ray analysis of family 39 beta-D-xylosidase from Geobacillus stearothermophilus T-6. Acta Crystallogr. D Biol. Crystallogr. 60 2004 583-5 [PubMed: 14993701] http://dx.doi.org/10.1107/S0907444904001088
	Yang JK, Yoon HJ, Ahn HJ, Lee BI, Pedelacq JD, Liong EC, Berendzen J, Laivenieks M, Vieille C, Zeikus GJ, Vocadlo DJ, Withers SG, Suh SW. Crystal structure of beta-D-xylosidase from Thermoanaerobacterium saccharolyticum, a family 39 glycoside hydrolase. J. Mol. Biol. 335 2004 155-65 [PubMed: 14659747] http://dx.doi.org/10.1016/j.jmb.2003.10.026
	Stoltzfus LJ, Sosa-Pineda B, Moskowitz SM, Menon KP, Dlott B, Hooper L, Teplow DB, Shull RM, Neufeld EF. Cloning and characterization of cDNA encoding canine alpha-L-iduronidase. mRNA deficiency in mucopolysaccharidosis I dog. J. Biol. Chem. 267 1992 6570-5 [PubMed: 1551868] http://intl.jbc.org/cgi/reprint/267/10/6570.pdf
	Henrissat B, Bairoch A. New families in the classification of glycosyl hydrolases based on amino acid sequence similarities. Biochem. J. 293 ( Pt 3) 1993 781-8 [PubMed: 8352747] http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=8352747&action=stream&blobtype=pdf
	Czjzek M, Ben David A, Bravman T, Shoham G, Henrissat B, Shoham Y. Enzyme-substrate complex structures of a GH39 beta-xylosidase from Geobacillus stearothermophilus. J. Mol. Biol. 353 2005 838-46 [PubMed: 16212978] http://dx.doi.org/10.1016/j.jmb.2005.09.003

InterPro 23.1