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InterPro: IPR000400 Glycoside hydrolase, family 46

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59 proteins

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Accession

IPR000400 Glyco_hydro_46

Type

Family

Signatures Help

Database	ID	Name	Proteins
Pfam	PF01374	Glyco_hydro_46	46
PIRSF	PIRSF036551	Chitosanase	37
PROSITE pattern	PS60000	CHITOSANASE_46_80	54
Signatures in BioMart

GO Term annotation Help

Process

GO:0005975 carbohydrate metabolic process

Function

GO:0016977 chitosanase activity

Component

GO:0005576 extracellular region

InterPro annotation

Entry Details in BioMart

Abstract

O-Glycosyl hydrolases EC:3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycosyl hydrolases, based on sequence similarity, has led to the definition of 85 different families [1, 2, 3]. This classification is available on the CAZy (CArbohydrate-Active EnZymes) web site [4]. Because the fold of proteins is better conserved than their sequences, some of the families can be grouped in clans.

Glycoside hydrolase family 46 GH46 comprises enzymes with only one known activity; chitosanase (EC:3.2.1.132).

Chitosanase enzymes catalyse the endohydrolysis of beta-1,4-linkages between N-acetyl-D-glucosamine and D-glucosamine residues in a partly acetylated chitosan.

Structural links Help

PDB - click here

SCOP: d.2.1.7

CATH: 1.20.141.10 , 3.30.386.10

Database links Help

PDBe-motif: PS60000

Enzyme: EC:3.2.1.132

CAZy: GH46

PROSITE doc: PDOC60000

PANDIT: PF01374

Blocks: IPB000400

Pfam Clan: CL0037.10

Taxonomic coverage Help

Example proteins Help

P33665 Chitosanase

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR000400	Glycoside hydrolase, family 46
	PDB Chain
	ModBase
	SCOP Domain
	CATH Domain

Publications Open in usermanual
1.	Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G. Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases. Proc. Natl. Acad. Sci. U.S.A. 92 7090-4 1995 [PubMed: 7624375] http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=7624375&action=stream&blobtype=pdf
2.	Davies G, Henrissat B. Structures and mechanisms of glycosyl hydrolases. Structure 3 853-9 1995 [PubMed: 8535779] http://dx.doi.org/10.1016/S0969-2126(01)00220-9
3.	Bairoch A. Classification of glycosyl hydrolase families and index of glycosyl hydrolase entries in SWISS-PROT. 1999
4.	Henrissat B, Coutinho PM. Carbohydrate-Active Enzymes server. 1999

Additional Reading Open in usermanual
	Marcotte EM, Monzingo AF, Ernst SR, Brzezinski R, Robertus JD. X-ray structure of an anti-fungal chitosanase from streptomyces N174. Nat. Struct. Biol. 3 1996 155-62 [PubMed: 8564542] http://dx.doi.org/10.1038/nsb0296-155
	Fukamizo T, Amano S, Yamaguchi K, Yoshikawa T, Katsumi T, Saito J, Suzuki M, Miki K, Nagata Y, Ando A. Bacillus circulans MH-K1 chitosanase: amino acid residues responsible for substrate binding. J. Biochem. 138 2005 563-9 [PubMed: 16272568] http://dx.doi.org/10.1093/jb/mvi156
	Henrissat B, Bairoch A. Updating the sequence-based classification of glycosyl hydrolases. Biochem. J. 316 ( Pt 2) 1996 695-6 [PubMed: 8687420] http://www.pubmedcentral.nih.gov/picrender.fcgi?tool=EBI&pubmedid=8687420&action=stream&blobtype=pdf
	Saito JI, Nagata Y, Ando A, Kita A, Miki K. Crystallization and preliminary X-ray crystallographic analysis of chitosanase from Bacillus circulans MH-K1. Acta Crystallogr. D Biol. Crystallogr. 51 1995 856-7 [PubMed: 15299827] http://dx.doi.org/10.1107/S090744499500268X
	Saito J, Kita A, Higuchi Y, Nagata Y, Ando A, Miki K. Crystal structure of chitosanase from Bacillus circulans MH-K1 at 1.6-A resolution and its substrate recognition mechanism. J. Biol. Chem. 274 1999 30818-25 [PubMed: 10521473] http://dx.doi.org/10.1074/jbc.274.43.30818
	Tremblay H, Blanchard J, Brzezinski R. A common molecular signature unifies the chitosanases belonging to families 46 and 80 of glycoside hydrolases. Can. J. Microbiol. 46 2000 952-5 [PubMed: 11068683] http://dx.doi.org/10.1139/cjm-46-10-952
	Fukamizo T, Brzezinski R. Chitosanase from Streptomyces sp. strain N174: a comparative review of its structure and function. Biochem. Cell Biol. 75 1997 687-96 [PubMed: 9599657] http://dx.doi.org/10.1139/bcb-75-6-687

InterPro 23.1