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InterPro: IPR000031 1-(5-Phosphoribosyl)-5-amino-4-imidazole-carboxylate (AIR) carboxylase

Protein matches Help

UniProtKB

Matches:

2120 proteins

Overview:	sorted by AC,	sorted by name,	of known structure,	proteins with splice variants
Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
Table:	For all matching proteins,	of known structure
Architectures
Accession List
Matches in BioMart

Accession

IPR000031 AIR_COase_core

Type

Domain

Signatures Help

Database	ID	Name	Proteins
Gene3D	G3DSA:3.40.50.7700	AIR_carboxyl	2029
Pfam	PF00731	AIRC	2111
PANTHER	PTHR23046	AIR_carboxyl	1756
SuperFamily	SSF52255	AIR_carboxyl	2107
TIGRFAMs	TIGR01162	purE	1749
Signatures in BioMart

InterPro Relationships Help

Found in

IPR016301 Phosphoribosylaminoimidazole carboxylase

GO Term annotation Help

Process

GO:0006189 'de novo' IMP biosynthetic process

Function

GO:0004638 phosphoribosylaminoimidazole carboxylase activity

InterPro annotation

Entry Details in BioMart

Abstract

Phosphoribosylaminoimidazole carboxylase is a fusion protein in plants and fungi, but consists of two non-interacting proteins in bacteria, PurK and PurE. PurK, N5-carboxyaminoimidazole ribonucleotide (N5_CAIR) synthetase, catalyzes the conversion of 5-aminoimidazole ribonucleotide (AIR), ATP, and bicarbonate to N5-CAIR, ADP, and Pi. PurE converts N5-CAIR to CAIR, the sixth step of de novo purine biosynthesis. In the presence of high concentrations of bicarbonate, PurE is reported able to convert AIR to CAIR directly and without ATP. Some members of this family contain two copies of this domain [1]. The crystal structure of PurE indicates a unique quaternary structure that confirms the octameric nature of the enzyme [2].

Structural links Help

PDB - click here

SCOP: c.23.8.1

CATH: 3.40.50.7700

Database links Help

Enzyme: EC:4.1.1.21

PANDIT: PF00731

Blocks: IPB000031

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR000031

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

P21264 Phosphoribosylaminoimidazole carboxylase

P22234 Multifunctional protein ADE2

Q10457 Probable multifunctional protein ADE2

Q9DCL9 Multifunctional protein ADE2

Q9I7S8 Multifunctional protein ADE2

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR013816	ATP-grasp fold, subdomain 2
IPR013817	Pre-ATP-grasp fold
IPR016301	Phosphoribosylaminoimidazole carboxylase
IPR001636	SAICAR synthetase
IPR016185	PreATP-grasp-like fold
IPR000031	1-(5-Phosphoribosyl)-5-amino-4-imidazole-carboxylate (AIR) carboxylase
IPR011761	ATP-grasp fold
IPR018236	SAICAR synthetase, conserved site
IPR011054	Rudiment single hybrid motif
IPR003135	ATP-grasp fold, ATP-dependent carboxylate-amine ligase-type
IPR005875	Phosphoribosylaminoimidazole carboxylase, ATPase subunit
	PDB Chain
	ModBase
	SWISS-MODEL

Publications Open in usermanual
1.	Meyer E, Kappock TJ, Osuji C, Stubbe J. Evidence for the direct transfer of the carboxylate of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to generate 4-carboxy-5-aminoimidazole ribonucleotide catalyzed by Escherichia coli PurE, an N5-CAIR mutase. Biochemistry 38 3012-8 1999 [PubMed: 10074353] http://dx.doi.org/10.1021/bi9827159
2.	Mathews II, Kappock TJ, Stubbe J, Ealick SE. Crystal structure of Escherichia coli PurE, an unusual mutase in the purine biosynthetic pathway. Structure 7 1395-406 1999 [PubMed: 10574791] http://dx.doi.org/10.1016/S0969-2126(00)80029-5

Additional Reading Open in usermanual
	Schwarzenbacher R, Jaroszewski L, von Delft F, Abdubek P, Ambing E, Biorac T, Brinen LS, Canaves JM, Cambell J, Chiu HJ, Dai X, Deacon AM, DiDonato M, Elsliger MA, Eshagi S, Floyd R, Godzik A, Grittini C, Grzechnik SK, Hampton E, Karlak C, Klock HE, Koesema E, Kovarik JS, Kreusch A, Kuhn P, Lesley SA, Levin I, McMullan D, McPhillips TM, Miller MD, Morse A, Moy K, Ouyang J, Page R, Quijano K, Robb A, Spraggon G, Stevens RC, van den Bedem H, Velasquez J, Vincent J, Wang X, West B, Wolf G, Xu Q, Hodgson KO, Wooley J, Wilson IA. Crystal structure of a phosphoribosylaminoimidazole mutase PurE (TM0446) from Thermotoga maritima at 1.77-A resolution. Proteins 55 2004 474-8 [PubMed: 15048837] http://dx.doi.org/10.1002/prot.20023
	Hoskins AA, Morar M, Kappock TJ, Mathews II, Zaugg JB, Barder TE, Peng P, Okamoto A, Ealick SE, Stubbe J. N5-CAIR mutase: role of a CO2 binding site and substrate movement in catalysis. Biochemistry 46 2007 2842-55 [PubMed: 17298082] http://dx.doi.org/10.1021/bi602436g
	Constantine CZ, Starks CM, Mill CP, Ransome AE, Karpowicz SJ, Francois JA, Goodman RA, Kappock TJ. Biochemical and structural studies of N5-carboxyaminoimidazole ribonucleotide mutase from the acidophilic bacterium Acetobacter aceti. Biochemistry 45 2006 8193-208 [PubMed: 16819818] http://dx.doi.org/10.1021/bi060465n
	Settembre EC, Chittuluru JR, Mill CP, Kappock TJ, Ealick SE. Acidophilic adaptations in the structure of Acetobacter aceti N5-carboxyaminoimidazole ribonucleotide mutase (PurE). Acta Crystallogr. D Biol. Crystallogr. 60 2004 1753-60 [PubMed: 15388921] http://dx.doi.org/10.1107/S090744490401858X
	Boyle MP, Kalliomaa AK, Levdikov V, Blagova E, Fogg MJ, Brannigan JA, Wilson KS, Wilkinson AJ. Crystal structure of PurE (BA0288) from Bacillus anthracis at 1.8 A resolution. Proteins 61 2005 674-6 [PubMed: 16138311] http://dx.doi.org/10.1002/prot.20599

InterPro 23.1