EBI Databases InterPro	Search Open in usermanual InterPro:
Jump to: InterProScan Databases Documentation FTP site Help Click on the icon for context sensitive help from the user manual. Advanced search

InterPro: IPR001537 tRNA/rRNA methyltransferase, SpoU

Protein matches Help

UniProtKB

Matches:

6734 proteins

Overview:	sorted by AC,	sorted by name,	of known structure,	proteins with splice variants
Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
Table:	For all matching proteins,	of known structure
Architectures
Accession List
Matches in BioMart

Accession

IPR001537 SpoU_MeTrfase

Type

Domain

Signatures Help

Database	ID	Name	Proteins
Pfam	PF00588	SpoU_methylase	6734
Signatures in BioMart

InterPro Relationships Help

Found in

IPR004384 RNA methyltransferase TrmH, group 1
IPR004440 RNA methyltransferase TrmH, group 2
IPR004441 RNA methyltransferase TrmH, group 3
IPR016479 tRNA/rRNA methyltransferase, YfiF, predicted
IPR016914 S-adenosyl-L-methionine dependent tRNA/rRNA methyltransferase, SpoU, predicted

GO Term annotation Help

Process

GO:0006396 RNA processing

Function

GO:0003723 RNA binding
GO:0008173 RNA methyltransferase activity

InterPro annotation

Entry Details in BioMart

Abstract

The spoU gene of Escherichia coli codes for a protein that shows strong similarities to previously characterised 2'-O-methyltransferases [1, 2]. The Pet56 protein of Saccharomyces cerevisiae has been shown to be required for ribose methylation at a universally conserved nucleotide in the peptidyl transferase centre of the mitochondrial large ribosomal RNA (21S rRNA). Cells reduced in this activity were deficient in formation of functional large subunits of the mitochondrial ribosome. The Pet56 protein catalyzes the site-specific formation of 2'-O-methylguanosine on in vitro transcripts of both mitochondrial 21S rRNA and E. coli 23S rRNA providing evidence for an essential modified nucleotide in rRNA [3].

Structural links Help

PDB - click here

SCOP: c.116.1.1

CATH: 3.40.1280.10

Database links Help

Enzyme: EC:2.1.1

PANDIT: PF00588

Blocks: IPB001537

Pfam Clan: CL0098.10

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR001537

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

P25270 rRNA methyltransferase, mitochondrial

P74261 Uncharacterized tRNA/rRNA methyltransferase slr1673

Q13395 Probable methyltransferase TARBP1

Q5ND52 RNA methyltransferase-like protein 1

Q9VW14 RNA methyltransferase-like protein 1 homolog

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR001537	tRNA/rRNA methyltransferase, SpoU
IPR013123	RNA 2-O ribose methyltransferase, substrate binding
IPR016024	Armadillo-type fold
IPR004441	RNA methyltransferase TrmH, group 3
	SWISS-MODEL
	PDB Chain
	ModBase

Publications Open in usermanual
1.	Persson BC, Jager G, Gustafsson C. The spoU gene of Escherichia coli, the fourth gene of the spoT operon, is essential for tRNA (Gm18) 2'-O-methyltransferase activity. Nucleic Acids Res. 25 4093-7 1997 [PubMed: 9321663] http://dx.doi.org/10.1093/nar/25.20.4093
2.	Koonin EV, Rudd KE. SpoU protein of Escherichia coli belongs to a new family of putative rRNA methylases. Nucleic Acids Res. 21 5519 1993 [PubMed: 8265370] http://dx.doi.org/10.1093/nar/21.23.5519
3.	Sirum-Connolly K, Mason TL. Functional requirement of a site-specific ribose methylation in ribosomal RNA. Science 262 1886-9 1993 [PubMed: 8266080] http://www.sciencemag.org/cgi/content/abstract/262/5141/1886

Additional Reading Open in usermanual
	Lim K, Zhang H, Tempczyk A, Krajewski W, Bonander N, Toedt J, Howard A, Eisenstein E, Herzberg O. Structure of the YibK methyltransferase from Haemophilus influenzae (HI0766): a cofactor bound at a site formed by a knot. Proteins 51 2003 56-67 [PubMed: 12596263] http://dx.doi.org/10.1002/prot.10323
	Pleshe E, Truesdell J, Batey RT. Structure of a class II TrmH tRNA-modifying enzyme from Aquifex aeolicus. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 61 2005 722-8 [PubMed: 16511140] http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=EBI&pubmedid=16511140
	Nureki O, Watanabe K, Fukai S, Ishii R, Endo Y, Hori H, Yokoyama S. Deep knot structure for construction of active site and cofactor binding site of tRNA modification enzyme. Structure 12 2004 593-602 [PubMed: 15062082] http://dx.doi.org/10.1016/j.str.2004.03.003
	Mosbacher TG, Bechthold A, Schulz GE. Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes. J. Mol. Biol. 345 2005 535-45 [PubMed: 15581897] http://dx.doi.org/10.1016/j.jmb.2004.10.051
	Nureki O, Shirouzu M, Hashimoto K, Ishitani R, Terada T, Tamakoshi M, Oshima T, Chijimatsu M, Takio K, Vassylyev DG, Shibata T, Inoue Y, Kuramitsu S, Yokoyama S. An enzyme with a deep trefoil knot for the active-site architecture. Acta Crystallogr. D Biol. Crystallogr. 58 2002 1129-37 [PubMed: 12077432] http://dx.doi.org/10.1107/S0907444902006601

InterPro 23.1