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InterPro: IPR000999 Ribonuclease III

Protein matches Help

UniProtKB

Matches:

3134 proteins

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Detailed:	sorted by AC,	sorted by name,	of known structure	proteins with splice variants
Table:	For all matching proteins,	of known structure
Architectures
Accession List
Matches in BioMart

Accession

IPR000999 RNase_III

Type

Domain

Signatures Help

Database	ID	Name	Proteins
Gene3D	G3DSA:1.10.1520.10	RNase_III	2315
Pfam	PF00636	Ribonuclease_3	2879
PROSITE pattern	PS00517	RNASE_3_1	1881
PROSITE profile	PS50142	RNASE_3_2	2420
SMART	SM00535	RIBOc	2838
SuperFamily	SSF69065	RNase_III	3104
Signatures in BioMart

InterPro Relationships Help

Found in

IPR008226 Uncharacterised conserved protein UCP005520
IPR011907 Ribonuclease III, bacterial

GO Term annotation Help

Process

GO:0006396 RNA processing

Function

GO:0003723 RNA binding
GO:0004525 ribonuclease III activity

InterPro annotation

Entry Details in BioMart

Abstract

Prokaryotic ribonuclease III (EC:3.1.26.3) (gene rnc) [1] is an enzyme that digests double-stranded RNA. It is involved in the processing of ribosomal RNA precursors and of some mRNAs. RNase III is evolutionary related to a number of proteins including [2]:

Saccharomyces cerevisiae (Baker's yeast) protein pac1, a ribonuclease that probably inhibits mating and meiosis by degrading a specific mRNA required for sexual development
yeast ribonuclease III (gene RNT1), a dsRNA-specific nuclease that cleaves eukaryotic preribosomal RNA at various sites
Caenorhabditis elegans hypothetical protein F26E4.13
Paramecium bursaria Chlorella virus 1 (PBCV-1) 1 protein A464R
Synechocystis sp. (strain PCC 6803) hypothetical protein slr0346
yeast hypothetical protein SpAC8A4.08c, a protein with a N-terminal helicase domain and a C-terminal RNase III domain
C. elegans hypothetical protein K12H4.8, a protein with the same structure as SpAC8A4.08c

Structural links Help

PDB - click here

SCOP: a.149.1.1

CATH: 1.10.1520.10

Database links Help

PDBe-motif: PS00517

Enzyme: EC:3.1.26.3

PROSITE doc: PDOC00448

PANDIT: PF00636

Blocks: IPB000999

Interactions Help

This domain has been experimentally proven to be involved in Protein:Protein interactions.
Representative data is shown with the following example proteins:

Taxonomic coverage Help

Overlapping InterPro entries Help

IPR000999

Numbers of overlapping proteins

Average numbers of overlapping amino acids

Example proteins Help

O01326 Ribonuclease 3

Q02555 Ribonuclease 3

Q8R418 Endoribonuclease Dicer

Q9UPY3 Endoribonuclease Dicer

Q9VCU9 Endoribonuclease Dcr-1

More proteins

Example Proteins Key

InterPro entry accession number/name and structure databases		Colour code
IPR005034	Dicer double-stranded RNA-binding fold
IPR003100	Argonaute/Dicer protein, PAZ
IPR001159	Double-stranded RNA binding
IPR014021	Helicase, superfamily 1/2, ATP-binding domain
IPR000999	Ribonuclease III
IPR001650	DNA/RNA helicase, C-terminal
IPR011545	DNA/RNA helicase, DEAD/DEAH box type, N-terminal
IPR014001	DEAD-like helicase, N-terminal
IPR014720	Double-stranded RNA-binding-like
	PDB Chain
	ModBase
	CATH Domain
	SWISS-MODEL
	SCOP Domain

Publications Open in usermanual
1.	Nashimoto H, Uchida H. DNA sequencing of the Escherichia coli ribonuclease III gene and its mutations. Mol. Gen. Genet. 201 25-9 1985 [PubMed: 3903434] http://dx.doi.org/10.1007/BF00397981
2.	Mian IS. Comparative sequence analysis of ribonucleases HII, III, II PH and D. Nucleic Acids Res. 25 3187-95 1997 [PubMed: 9241229] http://dx.doi.org/10.1093/nar/25.16.3187

Additional Reading Open in usermanual
	Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X. Intermediate states of ribonuclease III in complex with double-stranded RNA. Structure 13 2005 1435-42 [PubMed: 16216575] http://dx.doi.org/10.1016/j.str.2005.06.014
	Blaszczyk J, Gan J, Tropea JE, Court DL, Waugh DS, Ji X. Noncatalytic assembly of ribonuclease III with double-stranded RNA. Structure 12 2004 457-66 [PubMed: 15016361] http://dx.doi.org/10.1016/j.str.2004.02.004
	Gan J, Shaw G, Tropea JE, Waugh DS, Court DL, Ji X. A stepwise model for double-stranded RNA processing by ribonuclease III. Mol. Microbiol. 67 2008 143-54 [PubMed: 18047582]
	Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X. Structural insight into the mechanism of double-stranded RNA processing by ribonuclease III. Cell 124 2006 355-66 [PubMed: 16439209] http://dx.doi.org/10.1016/j.cell.2005.11.034
	Tahbaz N, Kolb FA, Zhang H, Jaronczyk K, Filipowicz W, Hobman TC. Characterization of the interactions between mammalian PAZ PIWI domain proteins and Dicer. EMBO Rep. 5 2004 189-94 [PubMed: 14749716] http://dx.doi.org/10.1038/sj.embor.7400070
	Blaszczyk J, Tropea JE, Bubunenko M, Routzahn KM, Waugh DS, Court DL, Ji X. Crystallographic and modeling studies of RNase III suggest a mechanism for double-stranded RNA cleavage. Structure 9 2001 1225-36 [PubMed: 11738048] http://dx.doi.org/10.1016/S0969-2126(01)00685-2

InterPro 23.1