EC - Δ32-enoyl-CoA isomerase

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IntEnz Enzyme Nomenclature


Accepted name:
Δ32-enoyl-CoA isomerase
Other names:
Δ3-cis2-trans-enoyl-CoA isomerase
acetylene-allene isomerase
dodecenoyl-CoA Δ-isomerase
dodecenoyl-CoA Δ3-cis2-trans-isomerase
Δ32-enoyl-CoA isomerase
3,2-trans-enoyl-CoA isomerase
ECI (gene name)
dodecenoyl-CoA isomerase
dodecenoyl-CoA (3Z)-(2E)-isomerase
Systematic name:
(3Z/3E)-alk-3-enoyl-CoA (2E)-isomerase



The enzyme participates in the β-oxidation of fatty acids with double bonds at an odd position. Processing of these substrates via the β-oxidation system results in intermediates with a cis- or trans-double bond at position C3, which cannot be processed further by the regular enzymes of the β-oxidation system. This enzyme isomerizes the bond to a trans bond at position C2, which can be processed further. The reaction rate is ten times higher for the (3Z) isomers than for (3E) isomers. The enzyme can also catalyse the isomerization of 3-acetylenic fatty acyl thioesters to 2,3-dienoyl fatty acyl thioesters.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004165
CAS Registry Number: 62213-29-0
UniProtKB/Swiss-Prot: (144) [show] [UniProt]


  1. Stoffel, W., Ditzer, R. and Caesar, H.
    Der Stoffwechsel der ungesättigten Fettsäuren. III. Zur β-Oxydation der Mono- und Polyenfettsäuren. Der Mechanismus der enzymatischen Reaktionen an Δ3cis-Enoyl-CoA-Verbindungen.
    Hoppe-Seyler's Z. Physiol. Chem. 339: 167-181 (1964). [PMID: 5830064]
  2. Stoffel, W. and Ecker, W.
    Δ3-cis,-Δ2-trans-Enoyl-CoA isomerase from rat liver mitochondria.
    Methods Enzymol. 14: 99-105 (1979).
  3. Stoffel, W. and Grol, M.
    Purification and properties of 3-cis-2-trans-enoyl-CoA isomerase (dodecenoyl-CoA Δ-isomerase) from rat liver mitochondria.
    Hoppe-Seyler's Z. Physiol. Chem. 359: 1777-1782 (1978). [PMID: 738702]
  4. Miesowicz, F. M., Bloch, K.
    Purification of hog liver isomerase. Mechanism of isomerization of 3-alkenyl and 3-alkynyl thioesters.
    J. Biol. Chem. 254: 5868-5877 (1979). [PMID: 376522]
  5. Engeland, K., Kindl, H.
    Purification and characterization of a plant peroxisomal delta 2, delta 3-enoyl-CoA isomerase acting on 3-cis-enoyl-CoA and 3-trans-enoyl-CoA.
    Eur. J. Biochem. 196: 699-705 (1991). [PMID: 2013292]
  6. Geisbrecht, B. V., Zhang, D., Schulz, H., Gould, S. J.
    Characterization of PECI, a novel monofunctional Delta(3), Delta(2)-enoyl-CoA isomerase of mammalian peroxisomes.
    J. Biol. Chem. 274: 21797-21803 (1999). [PMID: 10419495]
  7. Zhang, D., Yu, W., Geisbrecht, B. V., Gould, S. J., Sprecher, H., Schulz, H.
    Functional characterization of Delta3,Delta2-enoyl-CoA isomerases from rat liver.
    J. Biol. Chem. 277: 9127-9132 (2002). [PMID: 11781327]
  8. Goepfert, S., Vidoudez, C., Tellgren-Roth, C., Delessert, S., Hiltunen, J. K., Poirier, Y.
    Peroxisomal Delta(3),Delta(2)-enoyl CoA isomerases and evolution of cytosolic paralogues in embryophytes.
    Plant J. 56: 728-742 (2008). [PMID: 18657232]

[EC created 1978, modified 1980, modified 2018]