EC 4.99.1.5 - Aliphatic aldoxime dehydratase

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IntEnz Enzyme Nomenclature
EC 4.99.1.5

Names

Accepted name:
aliphatic aldoxime dehydratase
Other names:
OxdA
aliphatic aldoxime hydro-lyase
Systematic name:
aliphatic aldoxime hydro-lyase (aliphatic-nitrile-forming)

Reaction

Cofactors

Comments:

The enzyme from Pseudomonas chlororaphis contains Ca2+ and protoheme IX, the iron of which must be in the form Fe(II) for activity. The enzyme exhibits a strong preference for aliphatic aldoximes, such as butyraldoxime and acetaldoxime, over aromatic aldoximes, such as pyridine-2-aldoxime, which is a poor substrate. No activity was found with the aromatic aldoximes benzaldoxime and pyridine-4-aldoxime.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0034013

References

  1. Oinuma, K., Hashimoto, Y., Konishi, K., Goda, M., Noguchi, T., Higashibata, H. and Kobayashi, M.
    Novel aldoxime dehydratase involved in carbon-nitrogen triple bond synthesis of Pseudomonas chlororaphis B23. Sequencing, gene expression, purification, and characterization.
    J. Biol. Chem. 278: 29600-29608 (2003). [PMID: 12773527]
  2. Xie, S.-X., Kato, Y., Komeda, H., Yoshida, S. and Asano, Y.
    A gene cluster responsible for alkylaldoxime metabolism coexisting with nitrile hydratase and amidase in Rhodococcus globerulus A-4.
    Biochemistry 42: 12056-12066 (2003). [PMID: 14556637]
  3. Kato, Y., Yoshida, S., Xie, S.-X. and Asano, Y.
    Aldoxime dehydratase co-existing with nitrile hydratase and amidase in the iron-type nitrile hydratase-producer Rhodococcus sp. N-771.
    J. Biosci. Bioeng. 97: 250-259 (2004). [PMID: 16233624]

[EC 4.99.1.5 created 2004]