EC 4.99.1.4 - Sirohydrochlorin ferrochelatase

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IntEnz Enzyme Nomenclature
EC 4.99.1.4

Names

Accepted name:
sirohydrochlorin ferrochelatase
Other names:
CysG
Met8P
SirB
sirohydrochlorin ferro-lyase [incorrect]
Systematic name:
siroheme ferro-lyase (sirohydrochlorin-forming)

Reaction

Comments:

This enzyme catalyses the third of three steps leading to the formation of siroheme from uroporphyrinogen III. The first step involves the donation of two S-adenosyl-L-methionine-derived methyl groups to carbons 2 and 7 of uroporphyrinogen III to form precorrin-2 (EC 2.1.1.107, uroporphyrin-III C-methyltransferase) and the second step involves an NAD+-dependent dehydrogenation to form sirohydrochlorin from precorrin-2 (EC 1.3.1.76, precorrin-2 dehydrogenase). In Saccharomyces cerevisiae, the last two steps are carried out by a single bifunctional enzyme, Met8p. In some bacteria, steps 1-3 are catalysed by a single multifunctional protein called CysG, whereas in Bacillus megaterium, three separate enzymes carry out each of the steps, with SirB being responsible for the above reaction.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0051266
UniProtKB/Swiss-Prot: (149) [show] [UniProt]

References

  1. Schubert, H.L., Raux, E., Brindley, A.A., Leech, H.K., Wilson, K.S., Hill, C.P. and Warren, M.J.
    The structure of Saccharomyces cerevisiae Met8p, a bifunctional dehydrogenase and ferrochelatase.
    EMBO J. 21: 2068-2075 (2002). [PMID: 11980703]
  2. Warren, M.J., Raux, E., Schubert, H.L. and Escalante-Semerena, J.C.
    The biosynthesis of adenosylcobalamin (vitamin B12).
    Nat. Prod. Rep. 19: 390-412 (2002). [PMID: 12195810]

[EC 4.99.1.4 created 2004]