EC 4.2.3.5 - Chorismate synthase

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IntEnz Enzyme Nomenclature
EC 4.2.3.5

Names

Accepted name:
chorismate synthase
Other names:
5-enolpyruvylshikimate-3-phosphate phospholyase
5-O-(1-carboxyvinyl)-3-phosphoshikimate phosphate-lyase
Systematic name:
5-O-(1-carboxyvinyl)-3-phosphoshikimate phosphate-lyase (chorismate-forming)

Reaction

Cofactor

Comments:

Requires FMN. The reaction goes via a radical mechanism that involves reduced FMN and its semiquinone (FMNH•). Shikimate is numbered so that the double-bond is between C-1 and C-2, but some earlier papers numbered the ring in the reverse direction.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00628
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004107
CAS Registry Number: 9077-07-0
UniProtKB/Swiss-Prot: (714) [show] [UniProt]

References

  1. Gaertner, F.H. and Cole, K.W.
    Properties of chorismate synthase in Neurospora crassa.
    J. Biol. Chem. 248: 4602-4609 (1973). [PMID: 4146266]
  2. Morell, H., Clark, M.J., Knowles, P.F. and Sprinson, D.B.
    The enzymic synthesis of chorismic and prephenic acids from 3-enolpyruvylshikimic acid 5-phosphate.
    J. Biol. Chem. 242: 82-90 (1967). [PMID: 4289188]
  3. Welch, G.R., Cole, K.W. and Gaertner, F.H.
    Chorismate synthase of Neurospora crassa: a flavoprotein.
    Arch. Biochem. Biophys. 165: 505-518 (1974). [PMID: 4155270]
  4. Bornemann, S., Lowe, D.J. and Thorneley, R.N.
    The transient kinetics of Escherichia coli chorismate synthase: substrate consumption, product formation, phosphate dissociation, and characterization of a flavin intermediate.
    Biochemistry 35: 9907-9916 (1996). [PMID: 8703965]
  5. Bornemann, S., Theoclitou, M.E., Brune, M., Webb, M.R., Thorneley, R.N. and Abell, C.
    A secondary β deuterium kinetic isotope effect in the chorismate synthase reaction.
    Bioorg. Chem. 28: 191-204 (2000). [PMID: 11034781]
  6. Osborne, A., Thorneley, R.N., Abell, C. and Bornemann, S.
    Studies with substrate and cofactor analogues provide evidence for a radical mechanism in the chorismate synthase reaction.
    J. Biol. Chem. 275: 35825-35830 (2000). [PMID: 10956653]

[EC 4.2.3.5 created 1978 as EC 4.6.1.4, modified 1983, transferred 2000 to EC 4.2.3.5, modified 2002]