EC - Pseudouridylate synthase

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IntEnz Enzyme Nomenclature


Accepted name:
pseudouridylate synthase
Other names:
5-ribosyluracil 5-phosphate synthetase
ψUMP synthetase
pseudouridine monophosphate synthetase
pseudouridylate synthetase
pseudouridylic acid synthetase
uracil hydrolyase
pseudouridine synthase
uracil hydro-lyase (adding D-ribose 5-phosphate)
pseudouridine-5'-phosphate glycosidase
Systematic name:
uracil hydro-lyase (adding D-ribose 5-phosphate; pseudouridine-5'-phosphate-forming)



The reaction it readily reversible. While the enzymes from Tetrahymena pyriformis and Agrobacterium tumefaciens produce pseudouridine 5'-phosphate the enzyme from Escherichia coli functions as a pseudouridine-5'-phosphate glycosidase in vivo [5].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00869 , PROSITE:PDOC00885
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004730
CAS Registry Number: 9023-35-2
UniProtKB/Swiss-Prot: (57) [show] [UniProt]


  1. Heinrikson, R.L. and Goldwasser, E.
    Studies on the biosynthesis of 5-ribosyluracil 5'-monophosphate in Tetrahymena pyriformis.
    J. Biol. Chem. 239: 1177-1187 (1964). [PMID: 4936431]
  2. Matsushita, T. and Davis, F.F.
    Studies on pseudouridylic acid synthetase from various sources.
    Biochim. Biophys. Acta 238: 165-173 (1971). [PMID: 4936431]
  3. Rensen, J.F., Matsushita, T., Chirikjian, J.G. and Davis, F.F.
    Enzymatic synthesis of deoxypseudouridylic acid and a study of certain of its properties.
    Biochim. Biophys. Acta 281: 481-487 (1972). [PMID: 4569284]
  4. Suzuki, T. and Hochater, R.M.
    On the biosynthesis of pseudouridine and of pseudouridylic acid in Agrobacterium tumefaciens.
    Can. J. Biochem. 44: 259-272 (1966). [PMID: 5942965]
  5. Preumont, A., Snoussi, K., Stroobant, V., Collet, J. F., Van Schaftingen, E.
    Molecular identification of pseudouridine-metabolizing enzymes.
    J. Biol. Chem. 283: 25238-25246 (2008). [PMID: 18591240]

[EC created 1978]