EC 4.2.1.59 - 3-hydroxyacyl-[acyl-carrier-protein] dehydratase

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IntEnz Enzyme Nomenclature
EC 4.2.1.59

Names

Accepted name:
3-hydroxyacyl-[acyl-carrier-protein] dehydratase
Other names:
fabZ (gene name)
fabA (gene name)
D-3-hydroxyoctanoyl-[acyl carrier protein] dehydratase
β-hydroxyoctanoyl-acyl carrier protein dehydrase
β-hydroxyoctanoyl thioester dehydratase
β-hydroxyoctanoyl-ACP-dehydrase
(3R)-3-hydroxyoctanoyl-[acyl-carrier-protein] hydro-lyase
(3R)-3-hydroxyoctanoyl-[acyl-carrier-protein] hydro-lyase (oct-2-enoyl-[acyl-carrier protein]-forming)
3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase
Systematic name:
(3R)-3-hydroxyoctanoyl-[acyl-carrier protein] hydro-lyase (trans-2-enoyl-[acyl-carrier protein]-forming)

Reaction

Comments:

This enzyme is responsible for the dehydration step of the dissociated (type II) fatty-acid biosynthesis system that occurs in plants and bacteria. The enzyme uses fatty acyl thioesters of ACP in vivo. Different forms of the enzyme may have preferences for substrates with different chain length. For example, the activity of FabZ, the ubiquitous enzyme in bacteria, decreases with increasing chain length. Gram-negative bacteria that produce unsaturated fatty acids, such as Escherichia coli, have another form (FabA) that prefers intermediate chain length, and also catalyses EC 5.3.3.14, trans-2-decenoyl-[acyl-carrier protein] isomerase. Despite the differences both forms can catalyse all steps leading to the synthesis of palmitate (C16:0). FabZ, but not FabA, can also accept unsaturated substrates [4].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0019171 , GO:0047451
CAS Registry Number: 9030-85-7
UniProtKB/Swiss-Prot: (773) [show] [UniProt]

References

  1. Mizugaki, M., Swindell, A.C. and Wkil, S.J.
    Intermediate- and long-chain β-hydroxyacyl-ACP dehydrases from E. coli fatty acid synthetase.
    Biochem. Biophys. Res. Commun. 33: 520-527 (1968). [PMID: 4881058]
  2. Sharma, A., Henderson, B. S., Schwab, J. M., Smith, J. L.
    Crystallization and preliminary X-ray analysis of beta-hydroxydecanoyl thiol ester dehydrase from Escherichia coli.
    J. Biol. Chem. 265: 5110-5112 (1990). [PMID: 2180957]
  3. Mohan, S., Kelly, T. M., Eveland, S. S., Raetz, C. R., Anderson, M. S.
    An Escherichia coli gene (FabZ) encoding (3R)-hydroxymyristoyl acyl carrier protein dehydrase. Relation to fabA and suppression of mutations in lipid A biosynthesis.
    J. Biol. Chem. 269: 32896-32903 (1994). [PMID: 7806516]
  4. Heath, R. J., Rock, C. O.
    Roles of the FabA and FabZ beta-hydroxyacyl-acyl carrier protein dehydratases in Escherichia coli fatty acid biosynthesis.
    J. Biol. Chem. 271: 27795-27801 (1996). [PMID: 8910376]

[EC 4.2.1.59 created 1972, modified 2012]