EC 4.2.1.47 - GDP-mannose 4,6-dehydratase

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IntEnz Enzyme Nomenclature
EC 4.2.1.47

Names

Accepted name:
GDP-mannose 4,6-dehydratase
Other names:
GDP-D-mannose 4,6-dehydratase
GDP-D-mannose dehydratase
GDPmannose 4,6-dehydratase
Gmd
guanosine 5'-diphosphate-D-mannose oxidoreductase
guanosine diphosphomannose 4,6-dehydratase
guanosine diphosphomannose oxidoreductase
GDP-mannose 4,6-hydro-lyase
GDP-mannose 4,6-hydro-lyase (GDP-4-dehydro-6-deoxy-D-mannose-forming)
Systematic name:
GDP-α-D-mannose 4,6-hydro-lyase (GDP-4-dehydro-6-deoxy-α-D-mannose-forming)

Reaction

Cofactor

Comments:

The bacterial enzyme requires bound NAD+. This enzyme forms the first step in the biosynthesis of GDP-α-D-rhamnose and GDP-α-L-fucose. In Aneurinibacillus thermoaerophilus L420-91T, this enzyme acts as a bifunctional enzyme, catalysing the above reaction as well as the reaction catalysed by EC 1.1.1.281, GDP-4-dehydro-6-deoxy-D-mannose reductase [5]. Belongs to the short-chain dehydrogenase/reductase enzyme family, having homologous structures and a conserved catalytic triad of Lys, Tyr and Ser/Thr residues [6].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0008446
CAS Registry Number: 37211-59-9
UniProtKB/Swiss-Prot: (21) [show] [UniProt]

References

  1. Elbein, A.D. and Heath, E.C.
    The biosynthesis of cell wall lipopolysaccharide in Escherichia coli. II. Guanosine diphosphate 4-keto-6-deoxy-D-mannose, an intermediate in the biosynthesis of guanosine diphosphate colitose.
    J. Biol. Chem. 240: 1926-1931 (1965). [PMID: 14299611]
  2. Liao, T.-H. and Barber, G.A.
    Purification of guanosine 5'-diphosphate D-mannose oxidoreductase from Phaseolus vulgaris.
    Biochim. Biophys. Acta 276: 85-93 (1972). [PMID: 5047712]
  3. Melo, A., Elliott, H. and Glaser, L.
    The mechanism of 6-deoxyhexose synthesis. I. Intramolecular hydrogen transfer catalyzed by deoxythymidine diphosphate D-glucose oxidoreductase.
    J. Biol. Chem. 243: 1467-1474 (1968). [PMID: 4869560]
  4. Sullivan, F.X., Kumar, R., Kriz, R., Stahl, M., Xu, G.Y., Rouse, J., Chang, X.J., Boodhoo, A., Potvin, B. and Cumming, D.A.
    Molecular cloning of human GDP-mannose 4,6-dehydratase and reconstitution of GDP-fucose biosynthesis in vitro.
    J. Biol. Chem. 273: 8193-8202 (1988). [PMID: 9525924]
  5. Kneidinger, B., Graninger, M., Adam, G., Puchberger, M., Kosma, P., Zayni, S. and Messner, P.
    Identification of two GDP-6-deoxy-D-lyxo-4-hexulose reductases synthesizing GDP-D-rhamnose in Aneurinibacillus thermoaerophilus L420-91T.
    J. Biol. Chem. 276: 5577-5583 (2001). [PMID: 11096116]
  6. Mulichak, A.M., Bonin, C.P., Reiter, W.D. and Garavito, R.M.
    Structure of the MUR1 GDP-mannose 4,6-dehydratase from Arabidopsis thaliana: implications for ligand binding and specificity.
    Biochemistry 41: 15578-15589 (2000). [PMID: 12501186]

[EC 4.2.1.47 created 1972, modified 2004]