EC 4.2.1.36 - Homoaconitate hydratase

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature
EC 4.2.1.36

Names

Accepted name:
homoaconitate hydratase
Other names:
cis-homoaconitase
homoaconitase
HACN
Lys4
LysF
2-hydroxybutane-1,2,4-tricarboxylate hydro-lyase [incorrect]
Systematic name:
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate hydro-lyase [(Z)-but-1-ene-1,2,4-tricarboxylate-forming]

Reaction

Cofactor

Comments:

Requires a [4Fe-4S] cluster for activity. The enzyme from the hyperthermophilic eubacterium Thermus thermophilus can catalyse the reaction shown above but cannot catalyse the previously described reaction, i.e. formation of homocitrate by hydration of cis-homoaconitate. The enzyme responsible for the conversion of cis-homoaconitate into homocitrate in T. thermophilus is unknown at present but the reaction can be catalysed in vitro using aconitate hydratase from pig (EC 4.2.1.3) [2].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00423
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004409
CAS Registry Number: 9030-68-6
UniProtKB/Swiss-Prot: (21) [show] [UniProt]

References

  1. Strassman, M. and Ceci, L.N.
    Enzymatic formation of cis-homoaconitic acid, an intermediate in lysine biosynthesis in yeast.
    J. Biol. Chem. 241: 5401-5407 (1966). [PMID: 5954805]
  2. Jia, Y., Tomita, T., Yamauchi, K., Nishiyama, M. and Palmer, D.R.J.
    Kinetics and product analysis of the reaction catalysed by recombinant homoaconitase from Thermus thermophilus.
    Biochem. J. 396: 479-485 (2006). [PMID: 16524361]
  3. Zabriskie, T.M. and Jackson, M.D.
    Lysine biosynthesis and metabolism in fungi.
    Nat. Prod. Rep. 17: 85-97 (2000). [PMID: 10714900]

[EC 4.2.1.36 created 1972, modified 2007]