EC 4.2.1.107 - 3α,7α,12α-trihydroxy-5β-cholest-24-enoyl-CoA hydratase

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IntEnz Enzyme Nomenclature
EC 4.2.1.107

Names

Accepted name:
3α,7α,12α-trihydroxy-5β-cholest-24-enoyl-CoA hydratase
Other names:
46 kDa hydratase 2
D-3-hydroxyacyl-CoA dehydratase
(24R,25R)-3α,7α,12α,24-tetrahydroxy-5β-cholestanoyl-CoA hydro-lyase
Systematic name:
(24R,25R)-3α,7α,12α,24-tetrahydroxy-5β-cholestanoyl-CoA hydro-lyase [(24E)-3α,7α,12α-trihydroxy-5β-cholest-24-enoyl-CoA-forming]

Reaction

Comments:

This enzyme forms part of the rat peroxisomal multifunctional enzyme perMFE-2, which also exhibits a dehydrogenase activity. The enzyme is involved in the β-oxidation of the cholesterol side chain in the cholic-acid-biosynthesis pathway.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0033989
UniProtKB/Swiss-Prot:

References

  1. Qin, Y.-M., Haapalainen, A.M., Conry, D., Cuebas, D.A., Hiltunen, J.K. and Novikov, D.K.
    Recombinant 2-enoyl-CoA hydratase derived from rat peroxisomal multifunctional enzyme 2: role of the hydratase reaction in bile acid synthesis.
    Biochem. J. 328: 377-382 (1998). [PMID: 9371691]
  2. Xu, R. and Cuebas, D.A.
    The reactions catalyzed by the inducible bifunctional enzyme of rat liver peroxisomes cannot lead to the formation of bile acids.
    Biochem. Biophys. Res. Commun. 221: 271-278 (1996). [PMID: 8619845]
  3. Kinoshita, T., Miyata, M., Ismail, S.M., Fujimoto, Y., Kakinuma, K., Kokawa, N.I. and Morisaki, M.
    Synthesis and determination of stereochemistry of four diastereoisomers at the C-24 and C-25 positions of 3α,7α,12α,24-tetrahydroxy-5β-cholestan-26-oic acid and cholic acid.
    Chem. Pharm. Bull. 36: 134-141 (1988).
  4. Fujimoto, Y., Kinoshita, T., Oya, I., Kakinuma, K., Ismail, S.M., Sonoda, Y., Sato, Y. and Morisaki, M.
    Non-stereoselective conversion of the four diastereoisomers at the C-24 and C-25 positions of 3α,7α,12α,24-tetrahydroxy-5β-cholestan-26-oic acid and cholic acid.
    Chem. Pharm. Bull. 36: 142-145 (1988).
  5. Kurosawa, T., Sato, M., Nakano, H., Fujiwara, M., Murai, T., Yoshimura, T. and Hashimoto, T.
    Conjugation reactions catalyzed by bifunctional proteins related to β-oxidation in bile acid biosynthesis.
    Steroids 66: 107-114 (2001). [PMID: 11146090]
  6. Russell, D.W.
    The enzymes, regulation, and genetics of bile acid synthesis.
    Annu. Rev. Biochem. 72: 137-174 (2003). [PMID: 12543708]

[EC 4.2.1.107 created 2005]