EC 4.1.99.14 - Spore photoproduct lyase

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IntEnz Enzyme Nomenclature
EC 4.1.99.14

Names

Accepted name:
spore photoproduct lyase
Other names:
SPL
SAM
SP lyase
SplB
SplG
Systematic name:
spore photoproduct pyrimidine-lyase

Reaction

Cofactors

Comments:

This enzyme is a member of the 'AdoMet radical' (radical SAM) family. The enzyme binds a [4Fe-4S] cluster. The cluster is coordinated by 3 cysteines and an exchangeable SAM molecule [3]. The 5'-deoxy-adenosine radical formed after electron transfer from the [4Fe-4S] cluster to the S-adenosyl-L-methionine, initiates the repair by abstracting the C-6 hydrogen of the spore photoproduct lesion. During the second part of the repair process the SAM molecule is regenerated [3].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
CAS Registry Number: 37290-70-3
UniProtKB/Swiss-Prot:

References

  1. Chandor, A., Berteau, O., Douki, T., Gasparutto, D., Sanakis, Y., Ollagnier-de-Choudens, S., Atta, M., Fontecave, M.
    Dinucleotide spore photoproduct, a minimal substrate of the DNA repair spore photoproduct lyase enzyme from Bacillus subtilis.
    J. Biol. Chem. 281: 26922-26931 (2006). [PMID: 16829676]
  2. Pieck, J.C., Hennecke, U., Pierik, A.J., Friedel, M.G. and Carell, T
    Characterization of a new thermophilic spore photoproduct lyase from Geobacillus stearothermophilus (SplG) with defined lesion containing DNA substrates.
    J. Biol. Chem. 281: 36317-36326 (2006). [PMID: 16968710]
  3. Buis, J. M., Cheek, J., Kalliri, E., Broderick, J. B.
    Characterization of an active spore photoproduct lyase, a DNA repair enzyme in the radical S-adenosylmethionine superfamily.
    J. Biol. Chem. 281: 25994-26003 (2006). [PMID: 16829680]
  4. Mantel, C., Chandor, A., Gasparutto, D., Douki, T., Atta, M., Fontecave, M., Bayle, P. A., Mouesca, J. M., Bardet, M.
    Combined NMR and DFT studies for the absolute configuration elucidation of the spore photoproduct, a UV-induced DNA lesion.
    J. Am. Chem. Soc. 130: 16978-16984 (2008). [PMID: 19012397]
  5. Silver, S. C., Chandra, T., Zilinskas, E., Ghose, S., Broderick, W. E., Broderick, J. B.
    Complete stereospecific repair of a synthetic dinucleotide spore photoproduct by spore photoproduct lyase.
    J. Biol. Inorg. Chem. 15: 943-955 (2010). [PMID: 20405152]

[EC 4.1.99.14 created 2009, modified 2010]