EC 3.5.1.93 - Glutaryl-7-aminocephalosporanic-acid acylase

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IntEnz Enzyme Nomenclature
EC 3.5.1.93

Names

Accepted name:
glutaryl-7-aminocephalosporanic-acid acylase
Other names:
7β-(4-carboxybutanamido)cephalosporanic acid acylase
cephalosporin C acylase
glutaryl-7-ACA acylase
CA
GCA
GA
cephalosporin acylase
glutaryl-7-aminocephalosporanic acid acylase
GL-7-ACA acylase
Systematic name:
(7R)-7-(4-carboxybutanamido)cephalosporanate amidohydrolase

Reaction

Comments:

Forms 7-aminocephalosporanic acid, a key intermediate in the synthesis of cephem antibiotics. It reacts only weakly with cephalosporin C.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0033968
UniProtKB/Swiss-Prot:

References

  1. Ishii, Y., Saito, Y., Fujimura, T., Sasaki, H., Noguchi, Y., Yamada, H., Niwa, M. and Shimomura, K.
    High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176.
    Eur. J. Biochem. 230: 773-778 (1995). [PMID: 7607251]
  2. Kinoshita, T., Tada, T., Saito, Y., Ishii, Y., Sato, A. and Murata, M.
    Crystallization and preliminary X-ray analysis of cephalosporin C acylase from Pseudomonas sp. strain N176.
    Acta Crystallogr. D Biol. Crystallogr. 56: 458-459 (2000). [PMID: 10739919]
  3. Monti, D., Carrea, G., Riva, S., Baldaro, E. and Frare, G.
    Characterization of an industrial biocatalyst: immobilized glutaryl-7-ACA acylase.
    Biotechnol. Bioeng. 70: 239-244 (2000). [PMID: 10972935]
  4. Kwon, T.H., Rhee, S., Lee, Y.S., Park, S.S. and Kim, K.H.
    Crystallization and preliminary X-ray diffraction analysis of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas sp. GK16.
    J. Struct. Biol. 131: 79-81 (2000). [PMID: 10945972]
  5. Kim, Y., Yoon, K.-H., Khang, Y., Turley, S. and Hol, W.G.J.
    The 2.0 Å crystal structure of cephalosporin acylase.
    Structure Fold. Des. 8: 1059-1068 (2000). [PMID: 11080627]
  6. Huang, X., Zeng, R., Ding, X., Mao, X., Ding, Y., Rao, Z., Xie, Y., Jiang, W. and Zhao, G.
    Affinity alkylation of the Trp-B4 residue of the β-subunit of the glutaryl 7-aminocephalosporanic acid acylase of Pseudomonas sp. 130.
    J. Biol. Chem. 277: 10256-10264 (2002). [PMID: 11782466]
  7. Kim, J.K., Yang, I.S., Rhee, S., Dauter, Z., Lee, Y.S., Park, S.S. and Kim, K.H.
    Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation.
    Biochemistry 42: 4084-4093 (2003). [PMID: 12680762]

[EC 3.5.1.93 created 2005]