EC 3.5.1.89 - N-acetylglucosaminylphosphatidylinositol deacetylase

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IntEnz Enzyme Nomenclature
EC 3.5.1.89

Names

Accepted name:
N-acetylglucosaminylphosphatidylinositol deacetylase
Other names:
N-acetyl-D-glucosaminylphosphatidylinositol acetylhydrolase
N-acetylglucosaminylphosphatidylinositol de-N-acetylase
GlcNAc-PI de-N-acetylase
GlcNAc-PI deacetylase
acetylglucosaminylphosphatidylinositol deacetylase
Systematic name:
6-(N-acetyl-α-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol acetylhydrolase

Reaction

Comments:

Involved in the second step of glycosylphosphatidylinositol (GPI) anchor formation in all eukaryotes. The enzyme appears to be composed of a single subunit (PIG-L in mammalian cells and GPI12 in yeast). In some species, the long-chain sn-1-acyl group of the phosphatidyl group is replaced by a long-chain alkyl or alk-1-enyl group.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0000225
UniProtKB/Swiss-Prot:

References

  1. Doering, T.L., Masteron, W.J., Englund, P.T. and Hart, G.W.
    Biosynthesis of the glycosyl phosphatidylinositol membrane anchor of the trypanosome variant surface glycoprotein. Origin of the non-acetylated glucosamine.
    J. Biol. Chem. 264: 11168-11173 (1989). [PMID: 2525555]
  2. Nakamura, N., Inoue, N., Watanabe, R., Takahashi, M., Takeda, J., Stevens, V.L. and Kinoshita, T.
    Expression cloning of PIG-L, a candidate N-acetylglucosaminyl-phosphatidylinositol deacetylase.
    J. Biol. Chem. 272: 15834-15840 (1997). [PMID: 9188481]
  3. Watanabe, R., Ohishi, K., Maeda, Y., Nakamura, N. and Kinoshita, T.
    Mammalian PIG-L and its yeast homologue Gpi12p are N-acetylglucosaminylphosphatidylinositol de-N-acetylases essential in glycosylphosphatidylinositol biosynthesis.
    Biochem. J. 339: 185-192 (1999). [PMID: 10085243]
  4. Smith, T.K, Crossman, A., Borissow, C.N., Paterson, M.J., Dix, A., Brimacombe, J.S. and Ferguson, M.A.J.
    Specificity of GlcNAc-PI de-N-acetylase of GPI biosynthesis and synthesis of parasite-specific suicide substrate inhibitors.
    EMBO J. 20: 3322-3332 (2001). [PMID: 11432820]

[EC 3.5.1.89 created 1992 as EC 3.1.1.69, transferred 2002 to EC 3.5.1.89, modified 2002]